Emergence of mcr-1 Gene in Colistin-Resistant Escherichia coli Isolates from Chicken in Chitwan, Nepal.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2024-04-12 DOI:10.1089/fpd.2023.0151

Ashik Tiwari, P. Poudel, Santosh Khanal, S. Lekhak, S. Adhikari, Ramesh Sharma Regmi, Supriya Sharma, Om Prakash Panta, T. Karki

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Abstract

The escalating prevalence of colistin-resistant Escherichia coli in poultry has emerged as a significant concern. This study aimed to assess the occurrence of the mcr-1 gene in colistin-resistant E. coli isolates from poultry samples. A cross-sectional study was conducted at National Avian Disease Investigation Laboratory, Nepal, on 210 chicken meat samples, including liver, heart, and spleen. E. coli was isolated and identified by conventional cultural methods. Antibiotic resistance pattern was assessed by the Kirby-Bauer disc diffusion method. The mcr-1 gene was detected by conventional polymerase chain reaction. The average viable count in chicken meat samples was log 6.01 CFU (colony-forming unit)/g, whereas the average coliform count was log 3.85 CFU/g. Coliforms were detected in at least one sample from 48.01% of total samples. The prevalence of E. coli in all meat samples was 39.52%. Liver accounted for the largest fraction of E. coli isolates (45.45%). Cefepime was the most effective antibiotic. Among all isolates, 45 (54.21%) were multidrug-resistant E. coli, 17 (20.48%) were colistin-resistant E. coli, and 11 (64.70%) harbored the mcr-1 gene. High prevalence of multidrug-resistant E. coli isolates, colistin-resistant isolates, and mcr-1 gene-carrying isolates indicates a serious concern, as it could potentially lead to colistin resistance in human pathogens through horizontal transfer of resistant genes from poultry to humans.

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尼泊尔奇特旺地区鸡肉中耐药大肠埃希菌 mcr-1 基因的出现。

耐大肠菌素大肠埃希氏菌在家禽中的流行率不断上升，已成为一个令人严重关切的问题。本研究旨在评估家禽样本中耐药大肠杆菌分离物中 mcr-1 基因的发生情况。尼泊尔国家禽病调查实验室对 210 份鸡肉样本（包括肝脏、心脏和脾脏）进行了横断面研究。采用传统的培养方法分离和鉴定了大肠杆菌。抗生素耐药性模式采用柯比鲍尔盘扩散法进行评估。用常规聚合酶链反应检测 mcr-1 基因。鸡肉样本中的平均存活菌落数为对数 6.01 CFU（菌落形成单位）/克，而平均大肠菌群数为对数 3.85 CFU/克。48.01% 的总样本中至少有一个样本检测到大肠菌群。大肠杆菌在所有肉类样本中的感染率为 39.52%。肝脏占大肠杆菌分离物的最大比例（45.45%）。头孢吡肟是最有效的抗生素。在所有分离株中，45 株（54.21%）为耐多种药物大肠杆菌，17 株（20.48%）为耐可乐定大肠杆菌，11 株（64.70%）携带 mcr-1 基因。耐多药大肠杆菌、耐大肠菌素大肠杆菌和携带 mcr-1 基因的大肠杆菌的高流行率令人严重关切，因为这可能通过耐药基因从家禽到人类的水平转移，导致人类病原体对大肠菌素产生耐药性。

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.