Identification and assessment of antifungal susceptibility of Candida species based on bronchoalveolar lavage in immunocompromised and critically ill patients

IF 1.3 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2024-04-15 DOI:10.18502/ijm.v16i2.15362

Robabeh Rezaei, Rasoul Aliannejad, M. Falahati, Zeinab Ghasemi, Mahtab Ashrafi-Khozani, M. Fattahi, Tandis Razavi, S. Farahyar

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引用次数: 0

Abstract

Background and Objectives: The presence of fungi in the respiratory tract as mycobiome, particularly Candida species (spp.), remains a serious problem due to increasing numbers of immunocompromised patients. The confirmed reliable ex- istence of these pathogens due to frequent colonization is essential. This investigation aimed to recognize Candida spp. among isolates from bronchoalveolar lavage of immunocompromised and critically ill patients and to evaluate their suscep- tibility to antimycotic drugs. Materials and Methods: Bronchoalveolar lavage fluid was collected from 161 hospitalized patients presenting with sus- pected respiratory fungal infection /colonization. The specimens were examined by standard molecular and mycological assays. Candida spp. were recognized with sequence assessment of the D1-D2 section of the large subunit ribosomal DNA. The susceptibility of Candida isolates to common antimycotic drugs was distinguished by standard broth microdilution. Results: Seventy-one clinical isolates of Candida spp. were recognized. Candida albicans was the most frequent, followed by C. glabrata, C. krusei (Pichia kudriavzevii), C. dubliniensis, C. parapsilosis, and C. tropicalis. We found 5.1% of C. albi- cans isolates and 8% of C. glabrata isolates to show resistance to fluconazole. The whole of the Candida spp. were sensitive to amphotericin B and caspofungin. Conclusion: This study demonstrated that C. albicans and C. glabrata are the most common isolates of bronchoalveolar lavage fluid in patients, and the drug susceptibility screening confirmed that amphotericin B and caspofungin are effective against Candida spp. but some C. glabrata and C. albicans isolates showed resistance to fluconazole.

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根据免疫力低下和重症患者支气管肺泡灌洗液鉴定和评估念珠菌的抗真菌敏感性

背景与目的：由于免疫力低下的患者人数不断增加，真菌，尤其是念珠菌（Candida species，spp.）作为真菌生物群在呼吸道中的存在仍然是一个严重的问题。由于这些病原体经常定植，因此确认其可靠存在至关重要。本研究旨在识别从免疫力低下和重症患者支气管肺泡灌洗液中分离出的念珠菌属，并评估它们对抗真菌药物的敏感性。材料与方法：从 161 名疑似呼吸道真菌感染/定植的住院患者中采集支气管肺泡灌洗液。标本采用标准的分子和真菌学检测方法进行检验。通过对大亚基核糖体 DNA 的 D1-D2 部分进行序列评估，确认了念珠菌属。通过标准肉汤微量稀释法确定念珠菌分离物对常见抗真菌药物的敏感性。研究结果确认了 71 个临床分离的念珠菌属。最常见的是白色念珠菌，其次是光滑念珠菌、克鲁塞念珠菌（Pichia kudriavzevii）、杜布林念珠菌、副丝状念珠菌和热带念珠菌。我们发现 5.1%的白念珠菌分离株和 8%的格拉布拉塔念珠菌分离株对氟康唑具有抗药性。所有念珠菌属都对两性霉素 B 和卡泊芬净敏感。结论这项研究表明，白念珠菌和绿念珠菌是患者支气管肺泡灌洗液中最常见的分离株，药物敏感性筛选证实两性霉素 B 和卡泊芬净对念珠菌属有效，但部分绿念珠菌和白念珠菌分离株对氟康唑产生耐药性。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.