Protective Effect of N-Acetylcysteine on Rooster Semen Cryopreservation.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-18 DOI:10.1089/bio.2023.0103

Farhad Hakimi, Mohamad Amir Karimi Torshizi, M. Hezavehei, M. Sharafi

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引用次数: 0

Abstract

Cryopreservation of avian semen is a useful reproductive technique in the poultry industry. However, during cooling, elevated reactive oxygen species (ROS) levels have destructive effects on both quality and function of thawed sperm. The aim of the current study is to investigate the antioxidant effects of N-acetylcysteine (NAC) during rooster semen cryopreservation. Semen samples were collected from ten Ross 308 broiler breeder roosters (32 weeks) and mixed. The mixed samples were divided into five equal parts and cryopreserved in Lake Buffer extender that contained different concentrations (0, 0.01, 0.1, 1, and 10 mM) of NAC. The optimum concentration of NAC was determined based on quality parameters of mobility, viability, membrane integrity, acrosome integrity, lipid peroxidation, and mitochondrial membrane potential after the freeze-thaw process. There was a higher percentage (p < 0.05) of total motility (TM) (60.9 ± 2.4%) and progressive motility (PM) (35.6 ± 1.9%) observed with the NAC-0.1 group compared to the other groups. Significantly higher percentages of viability (74.4 ± 2.3% and 71 ± 2.3%), membrane integrity (76.4 ± 1.5% and 74.7 ± 1.5%) and mitochondrial membrane potential (67.1 ± 1.6% and 66.3 ± 1.6%) were observed in the NAC-0.1 and NAC-1 groups compared to the other frozen groups (p < 0.05). The lowest percentage of lipid peroxidation and nonviable sperm was found in the NAC-0.1 and NAC-1 groups compared to the other groups (p < 0.05). The average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), and acrosome integrity, were not affected by different concentrations of NAC in the thawed sperm (p > 0.05). Both NAC-0.1 and NAC-1 appear to be beneficial for maintaining the quality of rooster sperm after thawing.

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N-乙酰半胱氨酸对公鸡精液冷冻保存的保护作用

禽类精液冷冻保存是家禽业中一项有用的繁殖技术。然而，在冷却过程中，活性氧（ROS）水平的升高会对解冻精子的质量和功能产生破坏性影响。本研究旨在调查 N-乙酰半胱氨酸（NAC）在公鸡精液冷冻保存过程中的抗氧化作用。研究人员从 10 只罗斯 308 肉用种公鸡（32 周）身上采集精液样本并混合。混合后的样本被分成五个等份，并在含有不同浓度（0、0.01、0.1、1 和 10 mM）NAC 的湖泊缓冲扩展剂中进行冷冻保存。根据冻融过程后的流动性、存活率、膜完整性、顶体完整性、脂质过氧化和线粒体膜电位等质量指标，确定了 NAC 的最佳浓度。与其他组相比，NAC-0.1 组观察到的总活力（TM）（60.9 ± 2.4%）和渐进活力（PM）（35.6 ± 1.9%）百分比更高（p < 0.05）。与其他冷冻组相比，NAC-0.1 和 NAC-1 组的存活率（74.4 ± 2.3% 和 71 ± 2.3%）、膜完整性（76.4 ± 1.5% 和 74.7 ± 1.5%）和线粒体膜电位（67.1 ± 1.6% 和 66.3 ± 1.6%）显著更高（p < 0.05）。与其他组相比，NAC-0.1 和 NAC-1 组的过氧化脂质和无活力精子比例最低（p < 0.05）。解冻精子的平均路径速度（VAP）、直线速度（VSL）、曲线速度（VCL）和顶体完整性不受不同浓度 NAC 的影响（p > 0.05）。NAC-0.1和NAC-1似乎都有利于保持解冻后公鸡精子的质量。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.