Detection of senescent cells in the mucosal healing process on type 2 diabetic rats after tooth extraction for biomaterial development.

IF 1.9 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Dental materials journal Pub Date : 2024-04-19 DOI:10.4012/dmj.2023-262

Chuyi Luo, Masato Nakagawa, Yoichi Sumi, Yasuhiko Matsushima, Mamoru Uemura, Yoshitomo Honda, Naoyuki Matsumoto

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Abstract

The delayed mucosal healing of tooth extraction sockets in diabetes has few known effective treatment strategies, and its underlying mechanism remains unknown. Senescent cells may play a pivotal role in this delay, given the well-established association between diabetes, senescent cells, and wound healing. Here, we demonstrated an increase in p21- or p16-positive senescent cells in the epithelial and connective tissues of extraction sockets in type 2 diabetic rats compared to those in control rats. Between 7 and 14 days after tooth extraction, a decrease in senescent cells and improvement in re-epithelialization failure were observed in the epithelium, while an increase in senescent cells and persistence of inflammation were observed in the connective tissue. These results suggest that cellular senescence may have been induced by diabetes and contributed to delayed mucosal healing by suppressing re-epithelization and persistent inflammation. These findings provide new targets for treatment using biomaterials, cells, and drugs.

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检测 2 型糖尿病大鼠拔牙后粘膜愈合过程中的衰老细胞，以开发生物材料。

糖尿病患者拔牙窝粘膜愈合延迟，目前已知的有效治疗策略很少，其根本机制仍不清楚。鉴于糖尿病、衰老细胞和伤口愈合之间的关联已得到证实，衰老细胞可能在这种延迟中扮演了关键角色。在这里，我们证实，与对照组大鼠相比，2 型糖尿病大鼠拔牙窝上皮和结缔组织中 p21 或 p16 阳性的衰老细胞有所增加。拔牙后 7 至 14 天，观察到上皮细胞中衰老细胞减少，再上皮化失败情况有所改善，而结缔组织中衰老细胞增加，炎症持续存在。这些结果表明，细胞衰老可能是由糖尿病诱发的，并通过抑制再上皮化和持续炎症而导致粘膜愈合延迟。这些发现为使用生物材料、细胞和药物进行治疗提供了新的目标。

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来源期刊

Dental materials journal 医学-材料科学：生物材料

CiteScore

4.60

自引率

4.00%

发文量

102

审稿时长

3 months

期刊介绍： Dental Materials Journal is a peer review journal published by the Japanese Society for Dental Materials and Devises aiming to introduce the progress of the basic and applied sciences in dental materials and biomaterials. The dental materials-related clinical science and instrumental technologies are also within the scope of this journal. The materials dealt include synthetic polymers, ceramics, metals and tissue-derived biomaterials. Forefront dental materials and biomaterials used in developing filed, such as tissue engineering, bioengineering and artificial intelligence, are positively considered for the review as well. Recent acceptance rate of the submitted manuscript in the journal is around 30%.