Advancements and Practical Considerations for Biophysical Research: Navigating the Challenges and Future of Super-resolution Microscopy

Chemical & Biomedical Imaging Pub Date : 2024-04-19 DOI:10.1021/cbmi.4c00019

Huanhuan Chen, Guangjie Yan, Meng-Hsuan Wen, Kameron N. Brooks, Yuteng Zhang, Pei-San Huang and Tai-Yen Chen*,

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Abstract

The introduction of super-resolution microscopy (SRM) has significantly advanced our understanding of cellular and molecular dynamics, offering a detailed view previously beyond our reach. Implementing SRM in biophysical research, however, presents numerous challenges. This review addresses the crucial aspects of utilizing SRM effectively, from selecting appropriate fluorophores and preparing samples to analyzing complex data sets. We explore recent technological advancements and methodological improvements that enhance the capabilities of SRM. Emphasizing the integration of SRM with other analytical methods, we aim to overcome inherent limitations and expand the scope of biological insights achievable. By providing a comprehensive guide for choosing the most suitable SRM methods based on specific research objectives, we aim to empower researchers to explore complex biological processes with enhanced precision and clarity, thereby advancing the frontiers of biophysical research.

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生物物理研究的进展和实际考虑因素：驾驭超分辨率显微镜的挑战与未来

超分辨率显微镜（SRM）的引入极大地推动了我们对细胞和分子动力学的理解，提供了以前我们无法企及的详细视角。然而，在生物物理研究中应用 SRM 却面临着诸多挑战。本综述探讨了有效利用 SRM 的关键方面，从选择合适的荧光团、制备样品到分析复杂的数据集。我们探讨了可提高 SRM 能力的最新技术进步和方法改进。我们强调 SRM 与其他分析方法的整合，旨在克服固有的局限性，扩大可获得的生物学见解的范围。通过为根据具体研究目标选择最合适的 SRM 方法提供全面指导，我们旨在使研究人员能够以更高的精度和清晰度探索复杂的生物过程，从而推进生物物理研究的前沿发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical & Biomedical Imaging 化学与生物成像-

CiteScore

1.00

自引率

0.00%

发文量

期刊介绍： Chemical & Biomedical Imaging is a peer-reviewed open access journal devoted to the publication of cutting-edge research papers on all aspects of chemical and biomedical imaging. This interdisciplinary field sits at the intersection of chemistry physics biology materials engineering and medicine. The journal aims to bring together researchers from across these disciplines to address cutting-edge challenges of fundamental research and applications.Topics of particular interest include but are not limited to:Imaging of processes and reactionsImaging of nanoscale microscale and mesoscale materialsImaging of biological interactions and interfacesSingle-molecule and cellular imagingWhole-organ and whole-body imagingMolecular imaging probes and contrast agentsBioluminescence chemiluminescence and electrochemiluminescence imagingNanophotonics and imagingChemical tools for new imaging modalitiesChemical and imaging techniques in diagnosis and therapyImaging-guided drug deliveryAI and machine learning assisted imaging