Bio-Oil Production from Fish Processing Waste Residues Using Oleaginous Rhodotorula sp. R1 After Conventional Oil Extraction

IF 3.1 3区工程技术 Q3 ENERGY & FUELS

BioEnergy Research Pub Date : 2024-04-22 DOI:10.1007/s12155-024-10749-0

Fernanda Dias De Ávila, Benedict C. Okeke, Josiane Pinheiro Farias, Marcela da Silva Afonso, Márcio Santos Silva, Flávio Anastácio de Oliveira Camargo, Fátima Menezes Bento, Simone Pieniz, Robson Andreazza

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Abstract

Fish waste is a major environmental pollution problem and requires costly treatment prior to disposal. Conversion of fish waste to economically important and eco-friendly products will make fishing and fish processing more valuable and sustainable. This study evaluated waste residues from fish processing waste subjected to conventional physical extraction of fish oil for single-cell oil production using oleaginous yeast. Potential application of the single-cell oil to produce biodiesel was evaluated. The treatment containing fish waste residue (5%, w/v) and glucose (20 g/L, w/v) displayed the highest rate (14%) of total lipid generation. The fish waste residue proved to be a good nitrogen source for the oleaginous yeast, Rhodotorula sp. R1. At 15% (w/v) fish waste residue and 20% (w/v) glucose amendment of the medium, the highest biomass production was observed. The yeast bio-oil has a lipid profile like vegetable oils and consists of mainly long-chain fatty acids (between C14 and C24) which are suitable for biodiesel production. The most abundant fatty acids were palmitic acid (C16:0), elaidic acid (C18:1n-9t), and stearic acid (C18:0). FTIR analysis of the transesterification reaction product using the yeast oil confirmed its conversion to biodiesel. Although glucose amendment of medium supported lipid accumulation, it can be replaced with wastes rich in sugars to decrease the cost of single-cell bio-oil production. Results indicate the potential secondary value of fish processing waste in the cultivation of oleaginous yeast for bio-oil and biodiesel production.

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利用传统榨油后的含油藻 R1 从鱼类加工废料中生产生物油

鱼类废弃物是一个主要的环境污染问题，在处理之前需要进行昂贵的处理。将鱼类废弃物转化为具有重要经济价值的环保产品，将使渔业和鱼类加工业更具价值和可持续性。本研究对鱼类加工废弃物中的残留物进行了评估，这些残留物采用传统的物理方法提取鱼油，利用含油酵母生产单细胞油。对单细胞油生产生物柴油的潜在应用进行了评估。含有鱼类废渣（5%，w/v）和葡萄糖（20 克/升，w/v）的处理显示出最高的总脂生成率（14%）。鱼类废渣被证明是含油酵母 Rhodotorula sp.在培养基中添加 15%（重量/体积）的鱼类废渣和 20%（重量/体积）的葡萄糖时，观察到的生物量产量最高。酵母生物油的脂质特征与植物油相似，主要由长链脂肪酸（介于 C14 和 C24 之间）组成，适合用于生产生物柴油。最丰富的脂肪酸是棕榈酸（C16:0）、烯丙基酸（C18:1n-9t）和硬脂酸（C18:0）。对使用酵母油的酯交换反应产物进行的傅立叶变换红外光谱分析证实，该产物已转化为生物柴油。虽然培养基中的葡萄糖添加剂支持脂质积累，但可以用富含糖类的废物代替，以降低单细胞生物油的生产成本。研究结果表明，鱼类加工废弃物在培养含油酵母生产生物油和生物柴油方面具有潜在的二次利用价值。

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来源期刊

BioEnergy Research ENERGY & FUELS-ENVIRONMENTAL SCIENCES

CiteScore

6.70

自引率

8.30%

发文量

174

审稿时长

3 months

期刊介绍： BioEnergy Research fills a void in the rapidly growing area of feedstock biology research related to biomass, biofuels, and bioenergy. The journal publishes a wide range of articles, including peer-reviewed scientific research, reviews, perspectives and commentary, industry news, and government policy updates. Its coverage brings together a uniquely broad combination of disciplines with a common focus on feedstock biology and science, related to biomass, biofeedstock, and bioenergy production.