Construction of dental pulp decellularized matrix by cyclic lavation combined with mechanical stirring and its proteomic analysis.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2024-04-23 DOI:10.1088/1748-605X/ad4245

Zhijun Zhang, Fei Bi, Yibing Huang, Weihua Guo

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引用次数: 0

Abstract

The decellularized matrixhas a great potential for tissue remodeling and regeneration; however, decellularization could induce host immune rejection due to incomplete cell removal or detergent residues, thereby posing significant challenges for its clinical application. Therefore, the selection of an appropriate detergent concentration, further optimization of tissue decellularization technique, increased of biosafety in decellularized tissues, and reduction of tissue damage during the decellularization procedures are pivotal issues that need to be investigated. Inthis study, we tested several conditions and determined that 0.1% Sodium dodecyl sulfate(SDS) and three decellularization cycles were the optimal conditions for decellularization of pulp tissue. Decellularization efficiency was calculated and the preparation protocol for dental pulp decellularization matrix (DPDM) was further optimized. To characterize the optimized DPDM, the microstructure, odontogenesis-related protein and fiber content were evaluated. Our results showed that the properties of optimized DPDM were superior to those of the non-optimized matrix. We also performed the 4D-Label-free quantitative proteomic analysis of DPDM and demonstrated the preservation of proteins from the natural pulp. This study provides a solid theoretical and experimental foundation for the potential application of DPDM in pulp regeneration.

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通过循环灌洗结合机械搅拌构建牙髓脱细胞基质及其蛋白质组分析。

脱细胞基质在组织重塑和再生方面具有巨大潜力；然而，脱细胞可能会因细胞去除不彻底或洗涤剂残留而诱发宿主免疫排斥反应，从而为其临床应用带来重大挑战。因此，选择合适的洗涤剂浓度、进一步优化组织脱细胞技术、提高脱细胞组织的生物安全性以及减少脱细胞过程中的组织损伤是需要研究的关键问题。在本研究中，我们测试了几种条件，确定0.1%十二烷基硫酸钠（SDS）和三个脱细胞周期是纸浆组织脱细胞的最佳条件。通过计算脱细胞效率，进一步优化了牙髓脱细胞基质（DPDM）的制备方案。为了表征优化后的牙髓脱细胞基质，我们对其微观结构、与牙髓发生相关的蛋白质和纤维含量进行了评估。结果表明，优化后的 DPDM 性能优于未优化的基质。我们还对 DPDM 进行了 4D 无标记定量蛋白质组学分析，证明其保留了天然牙髓中的蛋白质。这项研究为 DPDM 在牙髓再生中的潜在应用提供了坚实的理论和实验基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567

期刊介绍： ACS Applied Electronic Materials is an interdisciplinary journal publishing original research covering all aspects of electronic materials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials science, engineering, optics, physics, and chemistry into important applications of electronic materials. Sample research topics that span the journal's scope are inorganic, organic, ionic and polymeric materials with properties that include conducting, semiconducting, superconducting, insulating, dielectric, magnetic, optoelectronic, piezoelectric, ferroelectric and thermoelectric. Indexed/Abstracted： Web of Science SCIE Scopus CAS INSPEC Portico