Bta-miR-365-3p-targeted FKBP5 participates in the AMPK/mTOR signaling pathway in the regulation of preadipocyte differentiation in cattle.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-04-24 DOI:10.5713/ab.23.0328

Mengdi Chen, Congcong Zhang, Zewen Wu, Siwei Guo, Wenfa Lv, Jixuan Song, Beibei Hao, Jinhui Bai, Xinxin Zhang, Hongyan Xu, Guangjun Xia

{"title":"Bta-miR-365-3p-targeted FKBP5 participates in the AMPK/mTOR signaling pathway in the regulation of preadipocyte differentiation in cattle.","authors":"Mengdi Chen, Congcong Zhang, Zewen Wu, Siwei Guo, Wenfa Lv, Jixuan Song, Beibei Hao, Jinhui Bai, Xinxin Zhang, Hongyan Xu, Guangjun Xia","doi":"10.5713/ab.23.0328","DOIUrl":null,"url":null,"abstract":"Objective\nMicroRNAs (miRNAs) are endogenous non-coding RNAs that can play a role in the post-transcriptional regulation of mammalian preadipocyte differentiation. However, the precise functional mechanism of its regulation of fat metabolism is not fully understood.\n\n\nMethods\nWe identified bta-miR-365-3p, which specifically targets the 3'untranslated region (3'UTR) of the FK506-binding protein 5 (FKBP5),and verified its mechanisms for regulating expression and involvement in adipogenesis.\n\n\nResults\nIn this study, we found that the overexpression of bta-miR-365-3p significantly decreased the lipid accumulation and triglyceride content in the adipocytes. Compared to inhibiting bta-miR-365-3p group, overexpression of bta-miR-365-3p can inhibit the expression of adipocyte differentiation-related genes C/EBPα and PPARγ. The dual-luciferase reporter system further validated the targeting relationship between bta-miR-365-3p and FKBP5. FKBP5 mRNA and protein expression were detected by qRT-PCR and Western blot. Overexpression of bta-miR-365-3p significantly down-regulated FKBP5 expression, while inhibition of bta-miR-365-3p showed the opposite, indicating that bta-miR-365-3p negatively regulates FKBP5. AMPK/mTOR signaling pathway is closely related to the regulation of cell growth and is involved in the development of bovine adipocytes. In this study, overexpression of bta-miR-365-3p significantly inhibited mRNA and protein expression of AMPK, mTOR, and SREBP1 genes, while the inhibition of bta-miR-365-3p expression was contrary to these results. Overexpression of FKBP5 significantly upregulated AMPK, mTOR, and SREBP1 gene expression, while inhibition of FKBP5 expression was contrary to the above experimental results.\n\n\nConclusion\nIn conclusion, these results indicate that bta-miR-365-3p may be involved in the AMPK/mTOR signaling pathway in regulating Yanbian yellow cattle preadipocytes differentiation by targeting the FKBP5 gene.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"48 6","pages":""},"PeriodicalIF":4.6000,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.5713/ab.23.0328","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}

引用次数: 0

Abstract

Objective MicroRNAs (miRNAs) are endogenous non-coding RNAs that can play a role in the post-transcriptional regulation of mammalian preadipocyte differentiation. However, the precise functional mechanism of its regulation of fat metabolism is not fully understood. Methods We identified bta-miR-365-3p, which specifically targets the 3'untranslated region (3'UTR) of the FK506-binding protein 5 (FKBP5),and verified its mechanisms for regulating expression and involvement in adipogenesis. Results In this study, we found that the overexpression of bta-miR-365-3p significantly decreased the lipid accumulation and triglyceride content in the adipocytes. Compared to inhibiting bta-miR-365-3p group, overexpression of bta-miR-365-3p can inhibit the expression of adipocyte differentiation-related genes C/EBPα and PPARγ. The dual-luciferase reporter system further validated the targeting relationship between bta-miR-365-3p and FKBP5. FKBP5 mRNA and protein expression were detected by qRT-PCR and Western blot. Overexpression of bta-miR-365-3p significantly down-regulated FKBP5 expression, while inhibition of bta-miR-365-3p showed the opposite, indicating that bta-miR-365-3p negatively regulates FKBP5. AMPK/mTOR signaling pathway is closely related to the regulation of cell growth and is involved in the development of bovine adipocytes. In this study, overexpression of bta-miR-365-3p significantly inhibited mRNA and protein expression of AMPK, mTOR, and SREBP1 genes, while the inhibition of bta-miR-365-3p expression was contrary to these results. Overexpression of FKBP5 significantly upregulated AMPK, mTOR, and SREBP1 gene expression, while inhibition of FKBP5 expression was contrary to the above experimental results. Conclusion In conclusion, these results indicate that bta-miR-365-3p may be involved in the AMPK/mTOR signaling pathway in regulating Yanbian yellow cattle preadipocytes differentiation by targeting the FKBP5 gene.

查看原文本刊更多论文

Bta-miR-365-3p 靶向 FKBP5 参与 AMPK/mTOR 信号通路对牛前脂肪细胞分化的调控。

目的微小RNA（miRNA）是一种内源性非编码RNA，可在哺乳动物前脂肪细胞分化的转录后调控中发挥作用。方法我们鉴定了特异性靶向 FK506 结合蛋白 5（FKBP5）3'非翻译区（3'UTR）的 bta-miR-365-3p，并验证了其调控表达和参与脂肪生成的机制。结果在这项研究中，我们发现过表达 bta-miR-365-3p 能显著减少脂肪细胞中的脂质积累和甘油三酯含量。与抑制 bta-miR-365-3p 组相比，过表达 bta-miR-365-3p 可抑制脂肪细胞分化相关基因 C/EBPα 和 PPARγ 的表达。双荧光素酶报告系统进一步验证了 bta-miR-365-3p 与 FKBP5 的靶向关系。通过 qRT-PCR 和 Western 印迹检测了 FKBP5 mRNA 和蛋白的表达。过表达bta-miR-365-3p会显著下调FKBP5的表达，而抑制bta-miR-365-3p则相反，表明bta-miR-365-3p对FKBP5有负向调控作用。AMPK/mTOR信号通路与细胞生长调控密切相关，参与了牛脂肪细胞的发育。在本研究中，过表达 bta-miR-365-3p 能显著抑制 AMPK、mTOR 和 SREBP1 基因的 mRNA 和蛋白表达，而抑制 bta-miR-365-3p 的表达则与上述结果相反。结论综上所述，这些结果表明，bta-miR-365-3p 可能通过靶向 FKBP5 基因参与了 AMPK/mTOR 信号通路对延边黄牛前脂肪细胞分化的调控。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.