Epidemiological cut-off values for Yersina ruckeri disc diffusion data generated by a standardised method.

IF 1.1 4区农林科学 Q3 FISHERIES

Diseases of aquatic organisms Pub Date : 2024-04-25 DOI:10.3354/dao03779

Peter Smith, L. Le Devendec, É. Jouy, Emeline Larvor, David Verner-Jeffreys, Andrew Wokorac Joseph, Elliot Stanton, Edel Light, Luana Cortinovis, T. Pretto, Amedeo Manfrin, P. Boitard, M. Jamin, Nicolas Keck, A. Le Breton, Benoit Thuillier, Christian Ravaille, S. Baron

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Abstract

In order to establish the meaning of data generated in antimicrobial agent susceptibility tests, it is necessary to develop internationally harmonised interpretive criteria. Currently, such criteria have not been developed for data generated in studies of the susceptibility of the fish pathogen Yersinia ruckeri. This work generated the data that would be required to set epidemiological cut-off values for the susceptibility data of this species that had been generated using a standardised disc diffusion method that specified the use of Mueller Hinton agar and incubation at 22°C for 24-28 h. Using this method, sets of inhibition zones data for 4 antimicrobial agents were generated by 3 independent laboratories. The data from these laboratories were aggregated and analysed using the statistically based normalised resistance interpretation. For ampicillin, florfenicol, oxytetracycline and trimethoprim-sulfamethoxazole the cut-off values calculated by this analysis were ≥16, ≥23, ≥24 and ≥30 mm, respectively. Evidence is presented demonstrating that the data for these 4 agents was of sufficient quantity and quality that they could be used by the relevant authorities to set internationally harmonised, consensus epidemiological cut-off values for Y. ruckeri.

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用标准化方法生成的罗氏耶尔森菌盘扩散数据的流行病学临界值。

为了确定抗菌剂药敏试验产生的数据的意义，有必要制定国际统一的解释标准。目前，还没有为鱼类病原体 Yersinia ruckeri 的药敏性研究中产生的数据制定此类标准。这项工作生成了为该物种药敏性数据设定流行病学临界值所需的数据，这些数据是使用标准化的盘扩散方法生成的，该方法规定使用穆勒欣顿琼脂，并在 22°C 孵育 24-28 小时。对这些实验室的数据进行汇总，并使用基于统计学的归一化耐药性解释进行分析。对于氨苄西林、氟苯尼考、土霉素和三甲双胍-磺胺甲噁唑，该分析计算出的临界值分别为≥16、≥23、≥24 和≥30 毫米。有证据表明，这 4 种制剂的数据在数量和质量上都足以供有关当局用于制定国际统一的、一致的拉氏酵母菌流行病学临界值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diseases of aquatic organisms 农林科学-兽医学

CiteScore

3.10

自引率

0.00%

发文量

审稿时长

8-16 weeks

期刊介绍： DAO publishes Research Articles, Reviews, and Notes, as well as Comments/Reply Comments (for details see DAO 48:161), Theme Sections and Opinion Pieces. For details consult the Guidelines for Authors. Papers may cover all forms of life - animals, plants and microorganisms - in marine, limnetic and brackish habitats. DAO''s scope includes any research focusing on diseases in aquatic organisms, specifically: -Diseases caused by coexisting organisms, e.g. viruses, bacteria, fungi, protistans, metazoans; characterization of pathogens -Diseases caused by abiotic factors (critical intensities of environmental properties, including pollution)- Diseases due to internal circumstances (innate, idiopathic, genetic)- Diseases due to proliferative disorders (neoplasms)- Disease diagnosis, treatment and prevention- Molecular aspects of diseases- Nutritional disorders- Stress and physical injuries- Epidemiology/epizootiology- Parasitology- Toxicology- Diseases of aquatic organisms affecting human health and well-being (with the focus on the aquatic organism)- Diseases as indicators of humanity''s detrimental impact on nature- Genomics, proteomics and metabolomics of disease- Immunology and disease prevention- Animal welfare- Zoonosis