Ameliorative effect of bofutsushosan (Fangfengtongshengsan) extract on the progression of aging-induced obesity

IF 2.5 4区医学 Q3 CHEMISTRY, MEDICINAL

Journal of Natural Medicines Pub Date : 2024-04-25 DOI:10.1007/s11418-024-01803-4

Takafumi Saeki, Saya Yamamoto, Junji Akaki, Takahiro Tanaka, Misaki Nakasone, Hidemasa Ikeda, Wei Wang, Makoto Inoue, Yoshiaki Manse, Kiyofumi Ninomiya, Toshio Morikawa

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Abstract

This study aimed to compare fat accumulation in young and aged mice raised on a high-fat diet and to characterize the obesity-reducing effects of a Kampo medicine, bofutsushosan (BTS; fangfengtongshengsan in Chinese). Aged mice fed a high-fat diet containing 2% BTS extract for 28 days exhibited a significant reduction in weight gain and accumulation of visceral and subcutaneous fat, which were greater degree of reduction than those of the young mice. When the treatment period was extended to two months, the serum aspartate aminotransferase and alanine aminotransferase levels and the accumulation of fat droplets in the hepatocytes decreased. The mRNA expression of mitochondrial uncoupling protein 1 (UCP1) in the brown adipose tissue was significantly reduced in the aged mice compared to the young mice but increased by 2% in the BTS-treated aged mice. Additionally, the effect of BTS extract on oleic acid–albumin-induced triglyceride accumulation in hepatoblastoma-derived HepG2 cells was significantly inhibited in a concentration-dependent manner. Evaluation of the single crude drug extracts revealed that Forsythia Fruit, Schizonepeta Spike, and Rhubarb were the active components in BTS extract. These results suggest that BTS extract is effective against visceral, subcutaneous, and ectopic fats in the liver, which tend to accumulate with aging. Thus, BTS extract is useful in preventing and ameliorating the development of obesity and metabolic syndrome.

Graphical Abstract

Abstract Image

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防风通圣散提取物对衰老引起的肥胖症的改善作用

本研究旨在比较以高脂肪饮食饲养的年轻小鼠和老年小鼠的脂肪积累情况，并描述一种康普药物--保福舒散（BTS；中文名称为防风通圣散）--的减少肥胖效果。用含有 2% BTS 提取物的高脂肪食物喂养老年小鼠 28 天后，小鼠的体重增加和内脏及皮下脂肪的积累均显著减少，而且减少程度高于年轻小鼠。当治疗期延长至两个月时，小鼠血清中的天冬氨酸氨基转移酶和丙氨酸氨基转移酶水平以及肝细胞中脂肪滴的积累均有所下降。与年轻小鼠相比，老龄小鼠棕色脂肪组织中线粒体解偶联蛋白 1（UCP1）的 mRNA 表达量明显减少，但 BTS 处理的老龄小鼠的 mRNA 表达量增加了 2%。此外，BTS 提取物对油酸-白蛋白诱导的肝母细胞瘤 HepG2 细胞甘油三酯积累的影响呈浓度依赖性明显抑制。对单一粗药物提取物的评估显示，连翘果、五味子穗和大黄是 BTS 提取物中的活性成分。这些结果表明，BTS 提取物对肝脏中的内脏脂肪、皮下脂肪和异位脂肪有效。因此，BTS 提取物可用于预防和改善肥胖症和代谢综合征的发展。

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来源期刊

Journal of Natural Medicines 医学-药学

CiteScore

6.90

自引率

3.00%

发文量

审稿时长

1.7 months

期刊介绍： The Journal of Natural Medicines is an international journal publishing original research in naturally occurring medicines and their related foods and cosmetics. It covers: -chemistry of natural products -biochemistry of medicinal plants -pharmacology of natural products and herbs, including Kampo formulas and traditional herbs -botanical anatomy -cultivation of medicinal plants. The journal accepts Original Papers, Notes, Rapid Communications and Natural Resource Letters. Reviews and Mini-Reviews are generally invited.