The expression of Catsup in the hindgut is essential for zinc homeostasis in Drosophila melanogaster

IF 2.3 2区农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Molecular Biology Pub Date : 2024-04-25 DOI:10.1111/imb.12916

Li Jin, Xueke Tian, Xiaowen Ji, Guiran Xiao

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Abstract

Zinc excretion is crucial for zinc homeostasis. However, the mechanism of zinc excretion has not been well characterized. Zinc homeostasis in Drosophila seems well conserved to mammals. In this study, we screened all members of the zinc transporters ZnT (SLC30) and Zip (SLC39) for their potential roles in Drosophila hindgut, an insect organ that belongs to the excretory system. The results indicated that Catecholamines up (Catsup, CG10449), a ZIP member localized to the Golgi, is responsible for zinc homeostasis in the hindgut of Drosophila hindgut-specific knockdown of Catsup leads to a developmental arrest in the larval stage, which could be rescued well by human ZIP7. Further study suggested that Catsup RNAi in the hindgut reduced zinc levels in the excretory system (containing the Malpighian tubule and hindgut) but exhibited systemic zinc overload. Besides, more calculi were observed in the Malpighian tubules of Catsup RNAi flies. The developmental arrest and calculi in the Malpighian tubules of hindgut-specific Catsup RNAi flies could be rescued by dietary zinc restriction but hypersensitivity to zinc. These results will help us understand the fundamental process of zinc excretion in higher eukaryotes.

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后肠中 Catsup 的表达对黑腹果蝇的锌平衡至关重要。

锌的排泄对锌的平衡至关重要。然而，锌的排泄机制尚未得到很好的描述。果蝇的锌平衡似乎与哺乳动物的锌平衡一致。在这项研究中，我们筛选了锌转运体 ZnT（SLC30）和 Zip（SLC39）的所有成员，研究它们在果蝇后肠（一种属于排泄系统的昆虫器官）中的潜在作用。结果表明，定位于高尔基体的ZIP成员儿茶酚胺（Catsup，CG10449）负责果蝇后肠的锌平衡，特异性敲除Catsup会导致果蝇幼虫期的发育停滞，而人类ZIP7可以很好地挽救这种停滞。进一步的研究表明，后肠中的 Catsup RNAi 会降低排泄系统（包括马尔皮希安小管和后肠）中的锌含量，但会表现出全身性锌超载。此外，在Catsup RNAi苍蝇的马尔皮希安小管中观察到更多的结石。后肠特异性Catsup RNAi蝇的发育停滞和马尔皮菅小管中的结石可以通过饮食锌限制而对锌超敏来挽救。这些结果将有助于我们了解高等真核生物锌排泄的基本过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Insect Molecular Biology 生物-昆虫学

CiteScore

4.80

自引率

3.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).