Echinococcus granulosus Sensu Stricto and Echinococcus multilocularis in a Gray Wolf (Canis lupus) in Turkey: Further Evidence for Increased Risk of Alveolar Echinococcosis in Urban Areas

IF 1.2 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2024-04-25 DOI:10.1007/s11686-024-00842-x

Muzaffer Akyuz, Ridvan Kirman, Esin Guven, Ibrahim Balkaya, Hamza Avcioglu

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引用次数: 0

Abstract

Objective

The aim of this study was to identify Echinococcus species by morphological and molecular means.

Methods

A dead gray wolf (Canis lupus) was found near Erzurum province and brought to the parasitology laboratory. Sedimentation and counting technique (SCT) and polymerase chain reaction (PCR) analysis were conducted.

Results

The SCT implications indicated that the wolf had a substantial worm burden (62,720 and 49,280 parasites) due to a co-infection of E. granulosus s.l. and E. multilocularis. Genus/species-specific PCR was used to analyze DNA extracted from adult worms and confirmed as E. granulosus s.s. and E. multilocularis, utilizing COI and 12S rRNA gene sequence analysis, respectively.

Conclusion

This report presents the first co-detection of E. granulosus s.s. and E. multilocularis in a gray wolf found in an urban area in a highly endemic area for human echinococcosis in northeastern Turkey. The results emphasize that AE is not only a problem of rural areas, but also occurs in urban areas, which may pose a threat to public health. Therefore, surveillance in urban areas is crucial. The need to develop new control strategies for domestic and wildlife in the study area is also highlighted.

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土耳其一只灰狼体内的严格棘球蚴和多角棘球蚴：城市地区肺泡棘球蚴病风险增加的进一步证据。

方法在埃尔祖鲁姆省附近发现一具灰狼（Canis lupus）尸体，并将其送到寄生虫学实验室。结果沉积和计数技术（SCT）和聚合酶链式反应（PCR）分析表明，这只狼身上有大量蠕虫（分别为 62720 和 49280 个寄生虫），这是因为它同时感染了肉毒圆线虫（E. granulosus s.l.）和多角体虫（E. multilocularis）。该报告首次在土耳其东北部人类棘球蚴病高度流行区的一个城市地区发现的灰狼体内同时检测到了颗粒棘球蚴和多角棘球蚴。研究结果表明，棘球蚴病不仅是农村地区的问题，也发生在城市地区，可能对公众健康构成威胁。因此，对城市地区进行监测至关重要。此外，还强调了为研究地区的家畜和野生动物制定新的控制策略的必要性。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.