MINFLUX nanoscopy: Visualising biological matter at the nanoscale level

IF 1.5 4区工程技术 Q3 MICROSCOPY

Journal of microscopy Pub Date : 2024-04-25 DOI:10.1111/jmi.13306

Alexander Carsten, A. Failla, Martin Aepfelbacher

引用次数: 0

Abstract

Since its introduction in 2017, MINFLUX nanoscopy has shown that it can visualise fluorescent molecules with an exceptional localisation precision of a few nanometres. In this overview, we provide a brief insight into technical implementations, fluorescent marker developments and biological studies that have been conducted in connection with MINFLUX imaging and tracking. We also formulate ideas on how MINFLUX nanoscopy and derived technologies could influence bioimaging in the future. This insight is intended as a general starting point for an audience looking for a brief overview of MINFLUX nanoscopy from theory to application.

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MINFLUX 纳米技术：纳米级生物物质可视化

自 2017 年推出以来，MINFLUX 纳米透视技术已经证明，它能够以几纳米的超高定位精度观察荧光分子。在本综述中，我们将简要介绍与 MINFLUX 成像和跟踪相关的技术实现、荧光标记开发和生物研究。我们还就 MINFLUX 纳米技术及其衍生技术在未来如何影响生物成像提出了一些想法。这一见解旨在为希望简要了解 MINFLUX 纳米透镜从理论到应用的读者提供一个总体出发点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microscopy 工程技术-显微镜技术

CiteScore

4.30

自引率

5.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit. The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens. Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.