The effect of cigarette and e-cigarette smoke on dental pulp stem cells proliferation capacity and differentiation [in vitro study]

IF 2.5 Q2 MULTIDISCIPLINARY SCIENCES

Beni-Suef University Journal of Basic and Applied Sciences Pub Date : 2024-04-26 DOI:10.1186/s43088-024-00496-8

Dalia Riad, Amani Nour Eldin, Mai Abdelhalim Hamouda

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引用次数: 0

Abstract

Background

Mesenchymal stem cells (MSCs) have long been known for their ability to regenerate tissue. Cigarette smoking is one environmental risk factor that may impair the performance of MSCs. Electronic cigarettes have recently become a popular and widely accepted alternative to tobacco cigarettes due to their safety. The present study aims to analyze how smoke extracts of cigarette tobacco and electronic cigarettes affect the capability of dental pulp stem cell (DPSCs) proliferation and osteogenic differentiation. In this study, DPSCs were isolated from healthy impacted third molars of non-smokers, and two smoke extracts were made from tobacco powder and electronic cigarettes. Half maximal inhibitory concentration (IC50) was calculated at two time intervals (14 and 21 days), and its effect on the proliferation and osteogenic differentiation of the DPSCs was assessed.

Results

The proliferation rate with the calculated IC50 of both smoke extracts was reduced compared to control cells. After 21 days of osteogenic induction, significantly fewer calcium deposits were visible among cells exposed to both smoke extracts. In addition, the expression of alkaline phosphatase and RANKL proteins was significantly reduced in differentiated DPSCs subjected to both smoke extracts.

Conclusions

DPSCs exposed to both smoke extracts showed decreased cell viability and osteogenic differentiation potentiality compared to control cells. Smoking in any form has a detrimental effect on the proliferation and regenerative capacity of MSCs.

查看原文本刊更多论文

香烟和电子烟烟雾对牙髓干细胞增殖能力和分化的影响［体外研究］

背景间充质干细胞（MSCs）因其再生组织的能力而久负盛名。吸烟是可能损害间充质干细胞性能的环境风险因素之一。最近，电子香烟因其安全性而成为一种流行并被广泛接受的烟草香烟替代品。本研究旨在分析香烟烟草和电子香烟的烟雾提取物如何影响牙髓干细胞（DPSCs）的增殖和成骨分化能力。本研究从非吸烟者的健康第三磨牙中分离出牙髓干细胞，并从烟草粉末和电子香烟中提取出两种烟雾提取物。结果与对照细胞相比，两种烟雾提取物计算出的 IC50 的增殖率均有所下降。诱导成骨 21 天后，暴露于两种烟雾提取物的细胞中可见的钙沉积明显减少。结论与对照细胞相比，暴露于两种烟雾提取物的 DPSCs 的细胞活力和成骨分化潜力都有所下降。任何形式的吸烟都会对间充质干细胞的增殖和再生能力产生不利影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Beni-Suef University Journal of Basic and Applied Sciences MULTIDISCIPLINARY SCIENCES-

CiteScore

2.60

自引率

0.00%

发文量

期刊介绍： Beni-Suef University Journal of Basic and Applied Sciences (BJBAS) is a peer-reviewed, open-access journal. This journal welcomes submissions of original research, literature reviews, and editorials in its respected fields of fundamental science, applied science (with a particular focus on the fields of applied nanotechnology and biotechnology), medical sciences, pharmaceutical sciences, and engineering. The multidisciplinary aspects of the journal encourage global collaboration between researchers in multiple fields and provide cross-disciplinary dissemination of findings.