Clinical feasibility of a label-free SERS assay for therapeutic drug monitoring of methotrexate

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research Pub Date : 2024-04-19 DOI:10.1016/j.sbsr.2024.100649

Elodie Dumont , Gohar Soufi , Yaman Göksel , Roman Slipets , Raheel Altaf Raja , Kjeld Schmiegelow , Kinga Zor , Anja Boisen

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引用次数: 0

Abstract

We present a therapeutic drug monitoring (TDM) feasibility study using a label-free surface-enhanced Raman spectroscopy (SERS)-based assay for quantifying methotrexate (MTX) from samples collected from children with acute lymphoblastic leukemia undergoing high-dose MTX therapy. We show that, when combined with an appropriate sample preparation (solid phase extraction) and multivariate data analysis (partial least squares regression), the SERS assay demonstrated a good correlation with a reference chromatographic method (r = 0.889, p < 0.005). We also found that the SERS-based approach underestimated MTX concentration, with a 10% bias calculated, but both methods showed similar variability (RSD of 8.4% for HPLC and 11.6% for SERS). The presented results are strong evidence that SERS can be used for TDM of MTX, and this work brings us one step closer to implementing a SERS-based assay in clinics.

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用于甲氨蝶呤治疗药物监测的无标记 SERS 分析法的临床可行性

我们介绍了一项治疗药物监测（TDM）可行性研究，该研究采用了一种基于无标记表面增强拉曼光谱（SERS）的检测方法，从接受大剂量 MTX 治疗的急性淋巴细胞白血病患儿样本中定量检测甲氨蝶呤（MTX）。我们的研究表明，结合适当的样品制备（固相萃取）和多元数据分析（偏最小二乘法回归），SERS 分析法与参考色谱法具有良好的相关性（r = 0.889，p < 0.005）。我们还发现，基于 SERS 的方法低估了 MTX 的浓度，计算出的偏差为 10%，但两种方法显示出相似的变异性（HPLC 的 RSD 为 8.4%，SERS 为 11.6%）。上述结果有力地证明了 SERS 可用于 MTX 的 TDM，这项工作使我们离在临床中使用基于 SERS 的检测方法更近了一步。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sensing and Bio-Sensing Research Engineering-Electrical and Electronic Engineering

CiteScore

10.70

自引率

3.80%

发文量

审稿时长

87 days

期刊介绍： Sensing and Bio-Sensing Research is an open access journal dedicated to the research, design, development, and application of bio-sensing and sensing technologies. The editors will accept research papers, reviews, field trials, and validation studies that are of significant relevance. These submissions should describe new concepts, enhance understanding of the field, or offer insights into the practical application, manufacturing, and commercialization of bio-sensing and sensing technologies. The journal covers a wide range of topics, including sensing principles and mechanisms, new materials development for transducers and recognition components, fabrication technology, and various types of sensors such as optical, electrochemical, mass-sensitive, gas, biosensors, and more. It also includes environmental, process control, and biomedical applications, signal processing, chemometrics, optoelectronic, mechanical, thermal, and magnetic sensors, as well as interface electronics. Additionally, it covers sensor systems and applications, µTAS (Micro Total Analysis Systems), development of solid-state devices for transducing physical signals, and analytical devices incorporating biological materials.