Polymorphisms in the cysteine dioxygenase gene and their association with taurine content in the Pacific oyster Crassostrea gigas

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-19 DOI:10.1016/j.cbpb.2024.110981

Xin Qiao , Ning Kong , Shiqing Sun , Xiang Li , Chunyu Jiang , Cong Luo , Lingling Wang , Linsheng Song

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Abstract

The Pacific oyster Crassostrea gigas is rich in taurine, which is crucial for its adaptation to the fluctuating intertidal environment and presents significant potential in improving taurine nutrition and boosting immunity in humans. Cysteine dioxygenase (CDO) is a key enzyme involved in the initial step of taurine biosynthesis and plays a crucial role in regulating taurine content in the body. In the present study, polymorphisms of CDO gene in C. gigas (CgCDO) and their association with taurine content were evaluated in 198 individuals. A total of 24 single nucleotide polymorphism (SNP) loci were identified in the exonic region of CgCDO gene by direct sequencing. Among these SNPs, c.279G>A and c.287C>A were found to be significantly associated with taurine content, with the GG and AA genotype at the two loci exhibiting enhanced taurine accumulation (p < 0.05). Haplotype analysis revealed that the 279GG/287AA haplotype had the highest taurine content of 29.24 mg/g, while the 279AA/287CC haplotype showed the lowest taurine content of 21.19 mg/g. These results indicated that the SNPs of CgCDO gene could influence the taurine content in C. gigas and have potential applications in the selective breeding of high-taurine varieties.

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半胱氨酸二氧酶基因的多态性及其与太平洋巨牡蛎牛磺酸含量的关系

太平洋牡蛎（Crassostrea gigas）含有丰富的牛磺酸，这对其适应潮间带变化无常的环境至关重要，并在改善牛磺酸营养和提高人体免疫力方面具有巨大潜力。半胱氨酸二氧酶（CDO）是参与牛磺酸生物合成初始步骤的关键酶，在调节体内牛磺酸含量方面起着至关重要的作用。本研究评估了 198 个个体的千层塔鱼 CDO 基因（CgCDO）多态性及其与牛磺酸含量的关系。通过直接测序，在 CgCDO 基因的外显子区域共鉴定出 24 个单核苷酸多态性（SNP）位点。在这些 SNP 位点中，c.279G>A 和 c.287C>A 与牛磺酸含量显著相关，这两个位点的 GG 和 AA 基因型表现出更强的牛磺酸积累（p <0.05）。单倍型分析表明，279GG/287AA单倍型的牛磺酸含量最高，为29.24毫克/克，而279AA/287CC单倍型的牛磺酸含量最低，为21.19毫克/克。这些结果表明，CgCDO 基因的 SNPs 可影响千岛湖牛磺酸的含量，在高牛磺酸品种的选育中具有潜在的应用价值。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.