Disruption of functions of primary human neonatal melanocytes cultured in the presence of bisphenol A and its analogs bisphenol F and bisphenol S

IF 6.6 Q1 ENGINEERING, ENVIRONMENTAL
Shilpi Goenka
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引用次数: 0

Abstract

Bisphenol A (BPA), bisphenol F (BPF), and bisphenol S (BPS) are contaminants of emerging concern (CECs) that humans are exposed to. In silico and zebrafish studies have linked BPA, BPF, and BPS exposure to pigmentation abnormalities, but no studies have examined their impact on primary human melanocytes. Herein, we examined the effects of BPA, BPF, and BPS exposure using human epidermal neonatal melanocytes. BPA induced the greatest cytotoxicity, followed by BPS, whereas BPF did not affect viability. BPA did not alter cellular melanin, whereas BPF and BPS diminished it at 100 µM. BPA decreased dendricity, as did BPF and BPS, although BPF was a potent suppressor of dendricity than BPS. BPA inhibited tyrosinase activity, followed by BPF, while BPS weakly suppressed tyrosinase activity. The tyrosinase activity was mostly recovered after the cessation of bisphenol treatments, although it remained lower for BPA and BPF. All bisphenols elevated cellular ROS production, although BPA and BPS showed non-monotonic dose responses. BPA and BPS augmented IL-6 cytokine secretion in melanocyte cultures treated with lipopolysaccharide, but BPF did not, suggesting that they exacerbate melanocyte inflammation. Collectively, these findings indicate BPA, BPF, and BPS, may impair melanocyte function and pose health hazards, warranting more study.

在双酚 A 及其类似物双酚 F 和双酚 S 存在的情况下培养的原代人类新生黑色素细胞的功能受到破坏
双酚 A(BPA)、双酚 F(BPF)和双酚 S(BPS)是人类接触到的新关注污染物(CECs)。硅学和斑马鱼研究表明,暴露于双酚 A、双酚 F 和双酚 S 会导致色素异常,但还没有研究探讨过它们对原发性人类黑色素细胞的影响。在此,我们利用人体表皮新生黑色素细胞研究了暴露于双酚 A、双酚 F 和双酚 S 的影响。双酚 A 诱导的细胞毒性最大,其次是双酚 BPS,而双酚 F 则不影响存活率。双酚 A不会改变细胞黑色素,而双酚 F 和双酚 S 在 100 µM 时会减少细胞黑色素。双酚 A 与双酚 F 和双酚 S 一样都会降低树突性,但双酚 F 比双酚 S 对树突性的抑制作用更强。双酚 A 可抑制酪氨酸酶活性,其次是双酚 F,而双酚 A 对酪氨酸酶活性的抑制作用较弱。停止双酚处理后,酪氨酸酶的活性基本恢复,但双酚 A 和双酚 F 的活性仍然较低。所有双酚都会增加细胞 ROS 的产生,但双酚 A 和双酚 BPS 显示出非单调剂量反应。经脂多糖处理的黑色素细胞培养物中,双酚 A 和双酚 BPS 会增加 IL-6 细胞因子的分泌,但双酚 F 不会,这表明它们会加剧黑色素细胞的炎症反应。总之,这些研究结果表明,双酚 A、双酚 F 和双酚 S 可能会损害黑色素细胞的功能,并对健康造成危害,因此值得进一步研究。
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来源期刊
Journal of hazardous materials letters
Journal of hazardous materials letters Pollution, Health, Toxicology and Mutagenesis, Environmental Chemistry, Waste Management and Disposal, Environmental Engineering
CiteScore
10.30
自引率
0.00%
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0
审稿时长
20 days
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