Oligodeoxynucleotides containing CpG motifs (CpG-ODN) restores immune regulatory functions of airway macrophages of patients with asthma

IF 4.9 3区医学 Q2 IMMUNOLOGY

Immunology Pub Date : 2024-04-18 DOI:10.1111/imm.13792

Huanping Zhang, Lihuan Wang, Aizhi Zhang, Xiangyu Wang, Yun Liao, Xiaoxue Chen, Xuejie Xu, Litao Yang, Yu Liu, Aifa Tang, Pingchang Yang

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Abstract

Allergic asthma is characterized by the polarization of Th2 cells and impaired immune regulation. Macrophages occupy the largest proportion of airway immune cells. This study aims to discover the mechanism that hinders the immune regulatory functions of airway macrophages. In this study, macrophages were isolated from cells in bronchoalveolar lavage fluids (BALF) collected from asthma patients and normal control (NC) subjects. The results indicated that macrophages occupied the largest portion of the cellular components in BALF. The frequency of IL-10⁺ macrophage was significantly lower in asthma patients than in NC subjects. The expression of IL-10 in macrophages of BALF was associated with the levels of asthma-related parameters. The immune-suppressive functions of BALF M0 cells were defective in asthma patients. The inducibility of IL-10 expression was impaired in BALF macrophages of asthma patients, which could be restored by exposing to CpG. In conclusion, the induction of IL-10 in macrophages of BALF in asthma patients was impaired, and it could be restored by exposure to CpG.

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含有 CpG 基序的寡脱氧核苷酸（CpG-ODN）可恢复哮喘患者气道巨噬细胞的免疫调节功能

过敏性哮喘的特点是 Th2 细胞极化和免疫调节功能受损。巨噬细胞在气道免疫细胞中所占比例最大。本研究旨在发现阻碍气道巨噬细胞发挥免疫调节功能的机制。本研究从哮喘患者和正常对照组（NC）的支气管肺泡灌洗液（BALF）细胞中分离出巨噬细胞。结果表明，巨噬细胞在 BALF 的细胞成分中占最大比例。哮喘患者中IL-10+巨噬细胞的频率明显低于NC受试者。BALF 中巨噬细胞中 IL-10 的表达与哮喘相关参数的水平有关。哮喘患者 BALF M0 细胞的免疫抑制功能存在缺陷。在哮喘患者的 BALF 巨噬细胞中，IL-10 的表达诱导能力受损，但通过暴露于 CpG 可以恢复。总之，哮喘患者 BALF 巨噬细胞中 IL-10 的诱导功能受损，而暴露于 CpG 可使其恢复。

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来源期刊

Immunology 医学-免疫学

CiteScore

11.90

自引率

1.60%

发文量

175

审稿时长

4-8 weeks

期刊介绍： Immunology is one of the longest-established immunology journals and is recognised as one of the leading journals in its field. We have global representation in authors, editors and reviewers. Immunology publishes papers describing original findings in all areas of cellular and molecular immunology. High-quality original articles describing mechanistic insights into fundamental aspects of the immune system are welcome. Topics of interest to the journal include: immune cell development, cancer immunology, systems immunology/omics and informatics, inflammation, immunometabolism, immunology of infection, microbiota and immunity, mucosal immunology, and neuroimmunology. The journal also publishes commissioned review articles on subjects of topical interest to immunologists, and commissions in-depth review series: themed sets of review articles which take a 360° view of select topics at the heart of immunological research.