Lipopolysaccharide-binding protein in follicular fluid is associated with the follicular inflammatory status and granulosa cell steroidogenesis in dairy cows

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-19 DOI:10.1262/jrd.2023-104

Fumie MAGATA, Misato KIKUZAWA, Heinrich BOLLWEIN, Fuko MATSUDA, Shingo HANEDA

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Abstract

Metabolic stress and subsequent hepatic dysfunction in high-producing dairy cows are associated with inflammatory diseases and declining fertility. Lipopolysaccharide (LPS)-binding protein (LBP) is produced by hepatocytes and controls the immune response, suggesting that it is involved in the pathophysiology of inflammation-related attenuation of reproductive functions during metabolic stress. This study investigated the effect of LBP on the inflammatory status, oocyte quality, and steroidogenesis in the follicular microenvironment of dairy cows. Using bovine ovaries obtained from a slaughterhouse, follicular fluid and granulosa cells were collected from large follicles to evaluate the follicular status of metabolism, inflammation, and steroidogenesis. Cumulus-oocyte complexes were aspirated from small follicles and subjected to in vitro embryo production. The results showed that follicular fluid LBP concentrations were significantly higher in cows with fatty livers and hepatitis than in those with healthy livers. Follicular fluid LBP and LPS concentrations were negatively correlated, whereas LPS concentration showed a positive correlation with the concentrations of non-esterified fatty acids (NEFA) and β-hydroxybutyric acid in follicular fluid. The blastulation rate of oocytes after in vitro fertilization was impaired in cows in which coexisting large follicles had high NEFA levels. Follicular fluid NEFA concentration was negatively correlated with granulosa cell expression of the estradiol (E₂) synthesis-related gene (CYP19A1). Follicular fluid LBP concentration was positively correlated with follicular fluid E₂ concentration and granulosa cell CYP19A1 expression. In conclusion, follicular fluid LBP may be associated with favorable conditions in the follicular microenvironment, including low LPS levels and high E₂ production by granulosa cells.

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卵泡液中的脂多糖结合蛋白与奶牛卵泡炎症状态和颗粒细胞类固醇生成有关

高产奶牛的代谢应激和随后的肝功能失调与炎症性疾病和繁殖力下降有关。肝细胞产生的脂多糖（LPS）结合蛋白（LBP）可控制免疫反应，这表明它参与了代谢应激期间与炎症相关的繁殖功能衰减的病理生理学过程。本研究调查了枸橼酸结合蛋白对奶牛卵泡微环境中炎症状态、卵母细胞质量和类固醇生成的影响。使用从屠宰场获得的牛卵巢，从大卵泡中采集卵泡液和颗粒细胞，以评估卵泡的新陈代谢、炎症和类固醇生成状况。从小卵泡中抽取卵泡液-卵母细胞复合物，并进行体外胚胎生产。结果显示，患有脂肪肝和肝炎的奶牛卵泡液中枸杞多糖的浓度明显高于肝脏健康的奶牛。卵泡液枸杞多糖浓度与 LPS 浓度呈负相关，而 LPS 浓度与卵泡液中的非酯化脂肪酸 (NEFA) 和 β-羟丁酸浓度呈正相关。在 NEFA 含量较高的共存大卵泡中，体外受精后卵母细胞的着床率会受到影响。卵泡液中的NEFA浓度与粒细胞中雌二醇（E2）合成相关基因（CYP19A1）的表达呈负相关。卵泡液 LBP 浓度与卵泡液 E2 浓度和颗粒细胞 CYP19A1 表达呈正相关。总之，卵泡液枸杞多糖可能与卵泡微环境中的有利条件有关，包括低LPS水平和颗粒细胞产生高E2。图表摘要全尺寸图片

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.