Correlating macroscopic plant growth parameters to nanomechanical properties of cellulose microfibrils

IF 5.4 Q1 PLANT SCIENCES
Nabila Masud , Jie Tang , Hasibul Hasan Hasib , Talukder (Zaki) Jubery , Xuan Xuan Lee , Hongqing Guo , Anwesha Sarkar
{"title":"Correlating macroscopic plant growth parameters to nanomechanical properties of cellulose microfibrils","authors":"Nabila Masud ,&nbsp;Jie Tang ,&nbsp;Hasibul Hasan Hasib ,&nbsp;Talukder (Zaki) Jubery ,&nbsp;Xuan Xuan Lee ,&nbsp;Hongqing Guo ,&nbsp;Anwesha Sarkar","doi":"10.1016/j.cpb.2024.100345","DOIUrl":null,"url":null,"abstract":"<div><p>The plant cell wall, a vital component in providing structural integrity and facilitating growth, comprises cellulose microfibrils among its major constituents. This study employed Atomic Force Microscopy (AFM) to investigate the intricate relationship between genetic mutation, cellulose microfibril organization, nanomechanical properties of cellulose microfibrils and plant growth. Focusing on the <em>Arabidopsis thaliana</em> wild type (WT) and <em>ixr1–2</em> mutant population (known for resistance to herbicide ISOXABEN), we utilized AFM to scrutinize cellulose microfibrils on the newly synthesized cell wall in 5-day-old dark-grown hypocotyls. Our macroscopic analysis revealed significant differences in plant growth, prompting a detailed examination at the nanoscale using AFM to discover if the macroscopic disparity between these two populations gets translated in structural details, orientation, and mechanical properties of cellulose microfibrils at the nanoscale too. AFM analysis highlighted distinct organizational disparities in cellulose microfibrils between the WT and mutant population. Our results revealed that the WT manifests a more aligned and oriented microfibril structure in contrast to the mutant population that shows significantly less aligned cellulose microfibrils in the plant growth direction. Also, the WT and mutant population demonstrate nuanced differences in height, width, roughness, deformation, and stiffness. The observed nanoscale alterations in microfibril structure and nano-mechanical properties contribute to an improved understanding of the intricate dynamics governing plant cell wall structure and its pivotal role in growth and development.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"38 ","pages":"Article 100345"},"PeriodicalIF":5.4000,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214662824000276/pdfft?md5=ae4fc8031bb21cc8c41c40baeb14e5ac&pid=1-s2.0-S2214662824000276-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Plant Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214662824000276","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

The plant cell wall, a vital component in providing structural integrity and facilitating growth, comprises cellulose microfibrils among its major constituents. This study employed Atomic Force Microscopy (AFM) to investigate the intricate relationship between genetic mutation, cellulose microfibril organization, nanomechanical properties of cellulose microfibrils and plant growth. Focusing on the Arabidopsis thaliana wild type (WT) and ixr1–2 mutant population (known for resistance to herbicide ISOXABEN), we utilized AFM to scrutinize cellulose microfibrils on the newly synthesized cell wall in 5-day-old dark-grown hypocotyls. Our macroscopic analysis revealed significant differences in plant growth, prompting a detailed examination at the nanoscale using AFM to discover if the macroscopic disparity between these two populations gets translated in structural details, orientation, and mechanical properties of cellulose microfibrils at the nanoscale too. AFM analysis highlighted distinct organizational disparities in cellulose microfibrils between the WT and mutant population. Our results revealed that the WT manifests a more aligned and oriented microfibril structure in contrast to the mutant population that shows significantly less aligned cellulose microfibrils in the plant growth direction. Also, the WT and mutant population demonstrate nuanced differences in height, width, roughness, deformation, and stiffness. The observed nanoscale alterations in microfibril structure and nano-mechanical properties contribute to an improved understanding of the intricate dynamics governing plant cell wall structure and its pivotal role in growth and development.

将植物宏观生长参数与纤维素微纤维的纳米力学特性联系起来
植物细胞壁是提供结构完整性和促进生长的重要组成部分,其主要成分包括纤维素微纤维。本研究采用原子力显微镜(AFM)研究基因突变、纤维素微纤维组织、纤维素微纤维的纳米力学性能和植物生长之间的复杂关系。我们以拟南芥野生型(WT)和ixr1-2突变体(因抗除草剂ISOXABEN而闻名)为研究对象,利用原子力显微镜仔细观察了5日龄暗生下胚轴新合成细胞壁上的纤维素微纤维。我们的宏观分析揭示了植物生长的显著差异,这促使我们使用原子力显微镜在纳米尺度上进行详细检查,以发现这两个群体之间的宏观差异是否也会在纳米尺度上转化为纤维素微纤维的结构细节、取向和机械特性。原子力显微镜分析凸显了 WT 和突变体群体之间纤维素微纤维在组织上的明显差异。我们的研究结果表明,WT 表现出更加整齐和定向的微纤维结构,相比之下,突变体群体在植物生长方向上的纤维素微纤维整齐度明显较低。此外,WT 和突变体在高度、宽度、粗糙度、变形和硬度方面也存在细微差别。观察到的微纤维结构和纳米力学性能的纳米级变化有助于更好地了解植物细胞壁结构的复杂动态及其在生长发育中的关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信