Spheroid construction strategies and application in 3D bioprinting

IF 8.1 1区 医学 Q1 ENGINEERING, BIOMEDICAL
Chunxiang Lu, Chuang Gao, Hao Qiao, Yi Zhang, Huazhen Liu, Aoxiang Jin, Yuanyuan Liu
{"title":"Spheroid construction strategies and application in 3D bioprinting","authors":"Chunxiang Lu, Chuang Gao, Hao Qiao, Yi Zhang, Huazhen Liu, Aoxiang Jin, Yuanyuan Liu","doi":"10.1007/s42242-024-00273-7","DOIUrl":null,"url":null,"abstract":"<p>Tissue engineering has been striving toward designing and producing natural and functional human tissues. Cells are the fundamental building blocks of tissues. Compared with traditional two-dimensional cultured cells, cell spheres are three-dimensional (3D) structures that can naturally form complex cell–cell and cell–matrix interactions. This structure is close to the natural environment of cells in living organisms. In addition to being used in disease modeling and drug screening, spheroids have significant potential in tissue regeneration. The 3D bioprinting is an advanced biofabrication technique. It accurately deposits bioinks into predesigned 3D shapes to create complex tissue structures. Although 3D bioprinting is efficient, the time required for cells to develop into complex tissue structures can be lengthy. The 3D bioprinting of spheroids significantly reduces the time required for their development into large tissues/organs during later cultivation stages by printing them with high cell density. Combining spheroid fabrication and bioprinting technology should provide a new solution to many problems in regenerative medicine. This paper systematically elaborates and analyzes the spheroid fabrication methods and 3D bioprinting strategies by introducing spheroids as building blocks. Finally, we present the primary challenges faced by spheroid fabrication and 3D bioprinting with future requirements and some recommendations.</p><h3 data-test=\"abstract-sub-heading\">Graphic abstract</h3>","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"188 1","pages":""},"PeriodicalIF":8.1000,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bio-Design and Manufacturing","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s42242-024-00273-7","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Tissue engineering has been striving toward designing and producing natural and functional human tissues. Cells are the fundamental building blocks of tissues. Compared with traditional two-dimensional cultured cells, cell spheres are three-dimensional (3D) structures that can naturally form complex cell–cell and cell–matrix interactions. This structure is close to the natural environment of cells in living organisms. In addition to being used in disease modeling and drug screening, spheroids have significant potential in tissue regeneration. The 3D bioprinting is an advanced biofabrication technique. It accurately deposits bioinks into predesigned 3D shapes to create complex tissue structures. Although 3D bioprinting is efficient, the time required for cells to develop into complex tissue structures can be lengthy. The 3D bioprinting of spheroids significantly reduces the time required for their development into large tissues/organs during later cultivation stages by printing them with high cell density. Combining spheroid fabrication and bioprinting technology should provide a new solution to many problems in regenerative medicine. This paper systematically elaborates and analyzes the spheroid fabrication methods and 3D bioprinting strategies by introducing spheroids as building blocks. Finally, we present the primary challenges faced by spheroid fabrication and 3D bioprinting with future requirements and some recommendations.

Graphic abstract

Abstract Image

球体构建策略及在三维生物打印中的应用
组织工程学一直致力于设计和制造天然的功能性人体组织。细胞是构成组织的基本单位。与传统的二维培养细胞相比,细胞球是三维(3D)结构,可以自然形成复杂的细胞-细胞和细胞-基质相互作用。这种结构接近于生物体内细胞的自然环境。除了用于疾病建模和药物筛选外,球形细胞在组织再生方面也具有巨大潜力。三维生物打印是一种先进的生物制造技术。它能将生物墨水准确地沉积到预先设计好的三维形状中,从而创建复杂的组织结构。虽然三维生物打印很高效,但细胞发育成复杂组织结构所需的时间可能很长。三维生物打印球体通过打印高密度细胞,大大缩短了细胞在后期培养阶段发育成大型组织/器官所需的时间。将球体制造与生物打印技术相结合,可为再生医学中的许多问题提供新的解决方案。本文通过引入球体作为构建模块,系统地阐述和分析了球体制造方法和三维生物打印策略。最后,我们提出了球形体制造和三维生物打印所面临的主要挑战以及未来的需求和一些建议。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Bio-Design and Manufacturing
Bio-Design and Manufacturing Materials Science-Materials Science (miscellaneous)
CiteScore
13.30
自引率
7.60%
发文量
148
期刊介绍: Bio-Design and Manufacturing reports new research, new technology and new applications in the field of biomanufacturing, especially 3D bioprinting. Topics of Bio-Design and Manufacturing cover tissue engineering, regenerative medicine, mechanical devices from the perspectives of materials, biology, medicine and mechanical engineering, with a focus on manufacturing science and technology to fulfil the requirement of bio-design.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信