The R2R3-MYB transcription factor ZeMYB32 negatively regulates anthocyanin biosynthesis in Zinnia elegans

IF 3.9 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Molecular Biology Pub Date : 2024-04-17 DOI:10.1007/s11103-024-01441-0

Lingli Jiang, Jiahong Chen, Jieyu Qian, Menghan Xu, Hongsheng Qing, Hefeng Cheng, Jianxin Fu, Chao Zhang

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Abstract

Anthocyanins are one of the important color-presenting substances in Zinnia elegans. In this study, an R2R3-MYB transcriptional factor ZeMYB32 was functionally characterized. Bioinformatic analysis indicated that ZeMYB32 belonged to the subgroup 4 and contained EAR repressor motif. The subcellular localization results showed that ZeMYB32 was localized on the nucleus. Stable transformation of ZeMYB32 in tobacco confirmed that ZeMYB32 significantly reduced the pigmentation of transgenic tobacco flowers and altered the flower phenotype, along with a decrease in the expression of several structural genes for anthocyanin synthesis. Yeast two-hybrid confirmed that ZeMYB32 could interact with ZeGL3, a key anthocyanin synthesis regulator from IIIf subgroup bHLH transcription factor. Moreover, transient transformation of tobacco leaves confirmed that the promoting effect of ZeMYB9 and ZeGL3 on anthocyanin synthesis was weakened by ZeMYB32, revealing that ZeMYB32 could compete with ZeMYB9 for binding to ZeGL3. Taken together, our results reveal that ZeMYB32 acts as a negative regulator of anthocyanin biosynthesis in Z. elegans.

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R2R3-MYB转录因子ZeMYB32负调控紫云英花青素的生物合成

花青素是紫云英中重要的呈色物质之一。本研究对R2R3-MYB转录因子ZeMYB32进行了功能表征。生物信息学分析表明，ZeMYB32 属于第 4 亚群，含有 EAR 抑制基团。亚细胞定位结果表明，ZeMYB32定位于细胞核。将 ZeMYB32 稳定转化于烟草后证实，ZeMYB32 能显著降低转基因烟草花的色素沉着，改变花的表型，同时降低多个花青素合成结构基因的表达。酵母双杂交证实，ZeMYB32 能与来自 IIIf 亚群 bHLH 转录因子的关键花青素合成调控因子 ZeGL3 相互作用。此外，烟草叶片的瞬时转化证实，ZeMYB9 和 ZeGL3 对花青素合成的促进作用被 ZeMYB32 削弱，这表明 ZeMYB32 可与 ZeMYB9 竞争与 ZeGL3 结合。综上所述，我们的研究结果表明 ZeMYB32 是秀丽隐杆线虫花青素生物合成的负调控因子。

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来源期刊

Plant Molecular Biology 生物-生化与分子生物学

自引率

2.00%

发文量

审稿时长

1.4 months

期刊介绍： Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.