OsATL32 ubiquitinates the reactive oxygen species-producing OsRac5–OsRbohB module to suppress rice immunity

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yuqing Yan, Hui Wang, Yan Bi, Jiajing Wang, Muhammad Noman, Dayong Li, Fengming Song
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引用次数: 0

Abstract

Ubiquitination-mediated protein degradation is integral to plant immunity, with E3 ubiquitin ligases acting as key factors in this process. Here, we report the functions of OsATL32, a plasma membrane-localized Arabidopsis Tóxicos En Levadura (ATL)-type E3 ubiquitin ligase, in rice (Oryza sativa) immunity and its associated regulatory network. We found that the expression of OsATL32 is downregulated in both compatible and incompatible interactions between rice and the rice blast fungus Magnaporthe oryzae. The OsATL32 protein level declines in response to infection by a compatible M. oryzae strain or to chitin treatment. OsATL32 negatively regulates rice resistance to blast and bacterial leaf blight diseases, as well as chitin-triggered immunity. Biochemical and genetic studies revealed that OsATL32 suppresses pathogen-induced reactive oxygen species (ROS) accumulation by mediating ubiquitination and degradation of the ROS-producing OsRac5–OsRbohB module, which enhances rice immunity against M. oryzae. The protein phosphatase PHOSPHATASE AND TENSIN HOMOLOG enhances rice blast resistance by dephosphorylating OsATL32 and promoting its degradation, preventing its negative effect on rice immunity. This study provides insights into the molecular mechanism by which the E3 ligase OsATL32 targets a ROS-producing module to undermine rice immunity.

Abstract Image

OsATL32 泛素化产生活性氧的 OsRac5-OsRbohB 模块,抑制水稻免疫
泛素化介导的蛋白质降解是植物免疫不可或缺的一部分,E3 泛素连接酶是这一过程中的关键因素。在这里,我们报告了质膜定位的拟南芥毒性连接酶(ATL)型 E3 泛素连接酶 OsATL32 在水稻(Oryza sativa)免疫中的功能及其相关调控网络。我们发现,在水稻与稻瘟病真菌 Magnaporthe oryzae 的相容和不相容相互作用中,OsATL32 的表达都会下调。OsATL32蛋白水平会随着相容的M. oryzae菌株感染或几丁质处理而下降。OsATL32 负向调节水稻对稻瘟病和细菌性叶枯病的抗性以及几丁质触发的免疫。生化和遗传研究发现,OsATL32 通过介导产生 ROS 的 OsRac5-OsRbohB 模块的泛素化和降解,抑制病原体诱导的活性氧(ROS)积累,从而增强水稻对 M. oryzae 的免疫力。蛋白磷酸酶 PHOSPHATASE AND TENSIN HOMOLOG 通过使 OsATL32 去磷酸化并促进其降解,防止其对水稻免疫的负面影响,从而增强水稻的抗稻瘟病能力。本研究揭示了 E3 连接酶 OsATL32 靶向 ROS 生成模块破坏水稻免疫的分子机制。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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