Development of microsatellite markers for the endangered butterfly Luehdorfia japonica Leech, 1889 (Lepidoptera: Papilionidae)

IF 0.7 4区 农林科学 Q4 ENTOMOLOGY
Shouhei Ueda, Chiaki Nakasuji, Naoyuki Nakahama, Norio Hirai, Minoru Ishii
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引用次数: 0

Abstract

The endangered butterfly species Luehdorfia japonica Leech, 1889 (Lepidoptera, Papilionidae) is endemic to the central and western parts of Honshu, Japan. This species inhabits deciduous forests and coppices, but areas of optimal habitats are decreasing due to the development of land and the abandonment of regular coppice management. We developed 17 microsatellite loci for L. japonica based on de novo genome sequence data and found that 16 of these loci exhibited polymorphisms in 34 individuals of L. japonica. In addition, polymorphisms of 15 of these microsatellite loci were observed in two individuals of L. puziloi. The number of alleles and the expected heterozygosity per locus in L. japonica were 2–11 and 0.11–0.83, respectively. A principal coordinate analysis based on this genetic information revealed genetic differentiation both within and among geographic populations of L. japonica. Thus, these microsatellite loci could potentially be useful for future conservation genetic studies, including monitoring the genetic diversity and population structure of this endangered butterfly species.

Abstract Image

为濒危蝴蝶 Luehdorfia japonica Leech, 1889(鳞翅目:凤蝶科)开发微卫星标记
濒危蝴蝶物种 Luehdorfia japonica Leech, 1889(鳞翅目,蝶科)是日本本州中部和西部的特有物种。该物种栖息于落叶林和灌木丛中,但由于土地开发和常规灌木丛管理的放弃,最佳栖息地的面积正在减少。我们基于全新的基因组序列数据,开发了 17 个 L. japonica 的微卫星位点,并发现其中 16 个位点在 34 个 L. japonica 个体中表现出多态性。此外,在 L. puziloi 的两个个体中也观察到了其中 15 个微卫星位点的多态性。在 L. japonica 中,每个位点的等位基因数和预期杂合度分别为 2-11 和 0.11-0.83。根据这些遗传信息进行的主坐标分析表明,L. japonica 地理种群内部和种群之间存在遗传分化。因此,这些微卫星位点可能有助于未来的保护遗传研究,包括监测这种濒危蝴蝶物种的遗传多样性和种群结构。
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来源期刊
Entomological Science
Entomological Science 生物-昆虫学
CiteScore
2.00
自引率
11.10%
发文量
30
审稿时长
6-12 weeks
期刊介绍: Entomological Science is the official English language journal of the Entomological Society of Japan. The Journal publishes original research papers and reviews from any entomological discipline or from directly allied field in ecology, behavioral biology, physiology, biochemistry, development, genetics, systematics, morphology, evolution and general entomology. Papers of applied entomology will be considered for publication if they significantly advance in the field of entomological science in the opinion of the Editors and Editorial Board.
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