Dynamically changed HSP70 after reperfusion following cerebral infarction in human and rats: correlation with p38 MAPK.

IF 1.6 4区医学 Q4 NEUROSCIENCES

Neuroreport Pub Date : 2024-03-27 DOI:10.1097/wnr.0000000000002022

Zhilan Tu, Pengpeng Jin, Qinghua Wang, Yanlin Feng, Xinjuan Chu, Lin Fu, Shuangxing Hou, Weiwei Li

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引用次数: 0

Abstract

We aimed to clarify the correlation between dynamic change of blood HSP70 and the prognosis of thrombolysis in human and rats, so as to explain the neuroprotection and early warning role of HSP70 in cerebral ischemia-reperfusion. Forty-two patients with acute ischemic stroke were divided into two groups according to the time from onset to thrombolytic therapy: 0 h-3 h (27 patients) and 3-4.5 h group (15 patients). The level of HSP70 in serum before and after thrombolysis was detected by ELISA. Furthermore, a rat model was also used to mimic the ischemic stroke and reperfusion. Peripheral blood of rat samples was collected to detect the level of HSP70 using Elisa. Several signal proteins from MAPK signaling pathway including JNK, p38, ERK (p42/44) were detected at different time points by Western blot of brain tissue. Patients who underwent thrombolytic therapy within 0-3 h had the highest HSP70 level at 1 h after thrombolysis. The higher HSP70 after thrombolysis, the better the patient prognosis. NIHSS scores showed HSP70 was positively correlated with cerebral ischemia. The levels of ERK family (p42/44 MAPK) and p-JNK were decreased gradually along with the time suffering cerebral ischemia. P-ERK, JNK, p-p38 had dynamic changes with increased ischemic time in the middle cerebral artery occlusion model. Dynamic change of HSP70 level in blood may be a biological index that reflects the functional condition of cell survival for cerebral ischemia and estimating the prognostic conditions. Importantly, HSP70 levels in blood were positively correlated with the p38 MAPK pathway in brain tissue.

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人和大鼠脑梗塞再灌注后 HSP70 的动态变化：与 p38 MAPK 的相关性。

我们旨在阐明人和大鼠血液中HSP70的动态变化与溶栓预后的相关性，从而解释HSP70在脑缺血再灌注中的神经保护和预警作用。42例急性缺血性脑卒中患者按发病至溶栓治疗的时间分为两组：0 h-3 h组（27例）和3-4.5 h组（15例）。采用 ELISA 法检测溶栓前后血清中 HSP70 的水平。此外，还使用大鼠模型模拟缺血性中风和再灌注。采集大鼠外周血样本，用 Elisa 检测 HSP70 的水平。通过对脑组织进行 Western 印迹，检测不同时间点 MAPK 信号通路的多个信号蛋白，包括 JNK、p38、ERK (p42/44)。在0-3小时内接受溶栓治疗的患者在溶栓后1小时的HSP70水平最高。溶栓后 HSP70 水平越高，患者的预后越好。NIHSS评分显示，HSP70与脑缺血呈正相关。ERK家族（p42/44 MAPK）和p-JNK的水平随着脑缺血时间的延长而逐渐降低。在大脑中动脉闭塞模型中，P-ERK、JNK和p-p38随着缺血时间的延长而发生动态变化。血液中HSP70水平的动态变化可能是反映脑缺血时细胞存活功能状况和估计预后条件的生物学指标。重要的是，血液中的HSP70水平与脑组织中的p38 MAPK通路呈正相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuroreport 医学-神经科学

CiteScore

3.20

自引率

0.00%

发文量

150

审稿时长

1 months

期刊介绍： NeuroReport is a channel for rapid communication of new findings in neuroscience. It is a forum for the publication of short but complete reports of important studies that require very fast publication. Papers are accepted on the basis of the novelty of their finding, on their significance for neuroscience and on a clear need for rapid publication. Preliminary communications are not suitable for the Journal. Submitted articles undergo a preliminary review by the editor. Some articles may be returned to authors without further consideration. Those being considered for publication will undergo further assessment and peer-review by the editors and those invited to do so from a reviewer pool. The core interest of the Journal is on studies that cast light on how the brain (and the whole of the nervous system) works. We aim to give authors a decision on their submission within 2-5 weeks, and all accepted articles appear in the next issue to press.