Ascorbic acid attenuates gasoline-induced testicular toxicity, sperm quality deterioration, and testosterone imbalance in rats

IF 1.7 4区医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Toxicology and Industrial Health Pub Date : 2024-04-10 DOI:10.1177/07482337241245154

Ogechukwu E Ezim, Joy Nyeche, Chisom E Nebeolisa, Chuka D Belonwu, Sunny O Abarikwu

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引用次数: 0

Abstract

The present study evaluated the protective effect of ascorbic acid (ASCB) against gasoline fumes (PET) induced testicular oxidative stress, sperm toxicity, and testosterone imbalance in Wistar rats. Twenty-four (24) male albino rats (75 ± 16 g) were randomized into three experimental groups ( N = 8). The control group: received normal saline, PET group: exposed to PET 6 h daily by inhalation in an exposure chamber and PET + 200 mg ASCB/kg body weight group: exposed to PET 6 h daily by inhalation and administered ASCB per os. Treatment of ASCB and PET exposure was done thrice and five times weekly for a period of 10 weeks respectively. ASCB co-treatment prevented PET-induced increases in the oxidative stress markers (glutathione, glutathione S-transferase, superoxide dismutase, catalase, hydrogen peroxide generation, nitric oxide, and lipid peroxidation) and serum testosterone concentration ( p < .05). Sperm quality was low and those with damaged heads and tails increased alongside histological injuries in the PET-exposed rats, which were also minimized with ASCB administration. ASCB protected against PET-induced oxidative stress, sperm, and testis damage in rats.

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抗坏血酸可减轻汽油引起的大鼠睾丸毒性、精子质量下降和睾酮失衡

本研究评估了抗坏血酸（ASCB）对汽油烟雾（PET）诱导的 Wistar 大鼠睾丸氧化应激、精子毒性和睾酮失衡的保护作用。将 24 只雄性白化大鼠（75 ± 16 克）随机分为三个实验组（N = 8）。对照组：接受普通生理盐水；PET 组：每天在暴露室中吸入 PET 6 小时；PET + 200 毫克 ASCB/kg 体重组：每天吸入 PET 6 小时，同时每只大鼠服用 ASCB。ASCB治疗和PET暴露每周分别进行三次和五次，为期10周。ASCB 联合治疗可防止 PET 诱导的氧化应激指标（谷胱甘肽、谷胱甘肽 S-转移酶、超氧化物歧化酶、过氧化氢生成、一氧化氮和脂质过氧化）和血清睾酮浓度的增加 ( p < .05)。PET 暴露大鼠的精子质量较低，头部和尾部受损的精子数量与组织学损伤同时增加，而服用 ASCB 可将这些损伤降至最低。ASCB 对 PET 诱导的大鼠氧化应激、精子和睾丸损伤有保护作用。

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来源期刊

Toxicology and Industrial Health 医学-毒理学

CiteScore

3.50

自引率

5.30%

发文量

审稿时长

4 months

期刊介绍： Toxicology & Industrial Health is a journal dedicated to reporting results of basic and applied toxicological research with direct application to industrial/occupational health. Such research includes the fields of genetic and cellular toxicology and risk assessment associated with hazardous wastes and groundwater.