Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model

IF 8.1 1区 医学 Q1 ENGINEERING, BIOMEDICAL
Sen Wang, Siqi Yao, Na Pei, Luge Bai, Zhiyan Hao, Dichen Li, Jiankang He, J. Miguel Oliveira, Xiaoyan Xue, Ling Wang, Xinggang Mao
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Abstract

Hypoxia is a typical feature of the tumor microenvironment, one of the most critical factors affecting cell behavior and tumor progression. However, the lack of tumor models able to precisely emulate natural brain tumor tissue has impeded the study of the effects of hypoxia on the progression and growth of tumor cells. This study reports a three-dimensional (3D) brain tumor model obtained by encapsulating U87MG (U87) cells in a hydrogel containing type I collagen. It also documents the effect of various oxygen concentrations (1%, 7%, and 21%) in the culture environment on U87 cell morphology, proliferation, viability, cell cycle, apoptosis rate, and migration. Finally, it compares two-dimensional (2D) and 3D cultures. For comparison purposes, cells cultured in flat culture dishes were used as the control (2D model). Cells cultured in the 3D model proliferated more slowly but had a higher apoptosis rate and proportion of cells in the resting phase (G0 phase)/gap I phase (G1 phase) than those cultured in the 2D model. Besides, the two models yielded significantly different cell morphologies. Finally, hypoxia (e.g., 1% O2) affected cell morphology, slowed cell growth, reduced cell viability, and increased the apoptosis rate in the 3D model. These results indicate that the constructed 3D model is effective for investigating the effects of biological and chemical factors on cell morphology and function, and can be more representative of the tumor microenvironment than 2D culture systems. The developed 3D glioblastoma tumor model is equally applicable to other studies in pharmacology and pathology.

Graphic abstract

Abstract Image

氧张力调节体外三维胶质母细胞瘤肿瘤模型中的细胞功能
缺氧是肿瘤微环境的一个典型特征,也是影响细胞行为和肿瘤进展的最关键因素之一。然而,由于缺乏能精确模拟天然脑肿瘤组织的肿瘤模型,阻碍了对缺氧对肿瘤细胞进展和生长影响的研究。本研究报告了通过将 U87MG(U87)细胞包裹在含有 I 型胶原蛋白的水凝胶中获得的三维(3D)脑肿瘤模型。研究还记录了培养环境中不同氧气浓度(1%、7% 和 21%)对 U87 细胞形态、增殖、活力、细胞周期、凋亡率和迁移的影响。最后,它对二维(2D)和三维培养进行了比较。为了便于比较,将在平培养皿中培养的细胞作为对照(二维模型)。与二维模型相比,三维模型培养的细胞增殖更慢,但凋亡率和处于静止期(G0 期)/间隙 I 期(G1 期)的细胞比例更高。此外,两种模式产生的细胞形态也有明显不同。最后,在三维模型中,缺氧(如 1%的氧气)会影响细胞形态、减缓细胞生长、降低细胞活力并增加细胞凋亡率。这些结果表明,构建的三维模型能有效研究生物和化学因素对细胞形态和功能的影响,而且比二维培养系统更能代表肿瘤微环境。所建立的三维胶质母细胞瘤肿瘤模型同样适用于其他药理学和病理学研究。
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来源期刊
Bio-Design and Manufacturing
Bio-Design and Manufacturing Materials Science-Materials Science (miscellaneous)
CiteScore
13.30
自引率
7.60%
发文量
148
期刊介绍: Bio-Design and Manufacturing reports new research, new technology and new applications in the field of biomanufacturing, especially 3D bioprinting. Topics of Bio-Design and Manufacturing cover tissue engineering, regenerative medicine, mechanical devices from the perspectives of materials, biology, medicine and mechanical engineering, with a focus on manufacturing science and technology to fulfil the requirement of bio-design.
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