Rapid monitoring of indoor airborne influenza and coronavirus with high air flowrate electrostatic sampling and PCR analysis

IF 6.1 2区 环境科学与生态学 Q2 ENGINEERING, ENVIRONMENTAL
Sanggwon An, Sangsoo Choi, Hyeong Rae Kim, Jungho Hwang
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Abstract

The World Health Organization has raised concerns about the possibility of airborne transmission in enclosed and poorly ventilated areas. Therefore, rapid monitoring of airborne viruses is necessary in multi-use facilities with dense population. Accordingly, an electrostatic air sampler (250 L/min) was developed in this work to obtain indoor viral aerosol samples for analysis via the Polymerase Chain Reaction (PCR). Aerosol tests with H1N1 and HCoV-229E were performed to evaluate the sample collection efficiency. PCR analysis, along with another aerosol test, was conducted to evaluate the recovery of the virus particles collected by the sampler. In laboratory tests, our electrostatic sampler obtained viral samples that were detectable by PCR under the simulated viral pandemic scenario (3000 RNA copies per cubic meter of air) within 40 min. The resulting cycle threshold (Ct) values were 35.07 and 37.1 for H1N1 and HCoV-229E, respectively. After the performance evaluation in the laboratory, field tests were conducted in a university classroom from October 28 to December 2, 2022. Influenza A and HCoV-229E were detected in two air samples, and the corresponding Ct values were 35.3 and 36.8. These PCR results are similar to those obtained from laboratory tests, considering the simulated viral pandemic scenario.

Abstract Image

利用高空气流速静电采样和 PCR 分析快速监测室内空气中的流感和冠状病毒
世界卫生组织对在封闭和通风不良的区域通过空气传播病毒的可能性表示担忧。因此,在人口密集的多用途设施中,有必要对空气传播的病毒进行快速监测。因此,本研究开发了一种静电空气采样器(250 升/分钟),用于获取室内病毒气溶胶样本,并通过聚合酶链式反应(PCR)进行分析。对 H1N1 和 HCoV-229E 进行了气溶胶测试,以评估样本收集效率。PCR 分析与另一项气溶胶测试一起进行,以评估采样器收集的病毒颗粒的回收率。在实验室测试中,我们的静电采样器在模拟病毒大流行的情况下(每立方米空气中 3000 个 RNA 拷贝),在 40 分钟内获得了可通过 PCR 检测的病毒样本。H1N1 和 HCoV-229E 的循环阈值 (Ct) 分别为 35.07 和 37.1。实验室性能评估结束后,2022 年 10 月 28 日至 12 月 2 日在一所大学的教室里进行了实地测试。在两个空气样本中检测到了甲型流感和 HCoV-229E,相应的 Ct 值分别为 35.3 和 36.8。考虑到模拟病毒大流行的情况,这些 PCR 结果与实验室检测结果相似。
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来源期刊
Frontiers of Environmental Science & Engineering
Frontiers of Environmental Science & Engineering ENGINEERING, ENVIRONMENTAL-ENVIRONMENTAL SCIENCES
CiteScore
10.90
自引率
12.50%
发文量
988
审稿时长
6.1 months
期刊介绍: Frontiers of Environmental Science & Engineering (FESE) is an international journal for researchers interested in a wide range of environmental disciplines. The journal''s aim is to advance and disseminate knowledge in all main branches of environmental science & engineering. The journal emphasizes papers in developing fields, as well as papers showing the interaction between environmental disciplines and other disciplines. FESE is a bi-monthly journal. Its peer-reviewed contents consist of a broad blend of reviews, research papers, policy analyses, short communications, and opinions. Nonscheduled “special issue” and "hot topic", including a review article followed by a couple of related research articles, are organized to publish novel contributions and breaking results on all aspects of environmental field.
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