The EIN3 transcription factor GmEIL1 improves soybean resistance to Phytophthora sojae

IF 4.8 1区农林科学 Q1 PLANT SCIENCES

Molecular plant pathology Pub Date : 2024-04-15 DOI:10.1111/mpp.13452

Xi Chen, Yan Sun, Yu Yang, Yuxin Zhao, Chuanzhong Zhang, Xin Fang, Hong Gao, Ming Zhao, Shengfu He, Bo Song, Shanshan Liu, Junjiang Wu, Pengfei Xu, Shuzhen Zhang

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Abstract

Phytophthora root and stem rot of soybean (Glycine max), caused by the oomycete Phytophthora sojae, is an extremely destructive disease worldwide. In this study, we identified GmEIL1, which encodes an ethylene‐insensitive3 (EIN3) transcription factor. GmEIL1 was significantly induced following P. sojae infection of soybean plants. Compared to wild‐type soybean plants, transgenic soybean plants overexpressing GmEIL1 showed enhanced resistance to P. sojae and GmEIL1‐silenced RNA‐interference lines showed more severe symptoms when infected with P. sojae. We screened for target genes of GmEIL1 and confirmed that GmEIL1 bound directly to the GmERF113 promoter and regulated GmERF113 expression. Moreover, GmEIL1 positively regulated the expression of the pathogenesis‐related gene GmPR1. The GmEIL1‐regulated defence response to P. sojae involved both ethylene biosynthesis and the ethylene signalling pathway. These findings suggest that the GmEIL1‐GmERF113 module plays an important role in P. sojae resistance via the ethylene signalling pathway.

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EIN3 转录因子 GmEIL1 可提高大豆对根瘤蚜的抗性

由卵菌 Phytophthora sojae 引起的大豆（Glycine max）茎腐病（Phytophthora root and stem rot）是一种在全球范围内破坏性极大的病害。在这项研究中，我们发现了编码乙烯不敏感3（EIN3）转录因子的GmEIL1。大豆植株感染 P. sojae 后，GmEIL1 被显著诱导。与野生型大豆植株相比，过表达 GmEIL1 的转基因大豆植株对 P. sojae 的抗性更强，而 GmEIL1 沉默的 RNA 干涉品系在感染 P. sojae 后症状更严重。我们筛选了 GmEIL1 的靶基因，证实 GmEIL1 可直接与 GmERF113 启动子结合并调控 GmERF113 的表达。此外，GmEIL1 还能正向调节致病相关基因 GmPR1 的表达。GmEIL1 调节的对 P. sojae 的防御反应涉及乙烯的生物合成和乙烯信号通路。这些研究结果表明，GmEIL1-GmERF113 模块通过乙烯信号途径在 P. sojae 的抗性中发挥了重要作用。

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来源期刊

Molecular plant pathology 生物-植物科学

CiteScore

9.40

自引率

4.10%

发文量

120

审稿时长

6-12 weeks

期刊介绍： Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.