Genomic variability within laboratory and wild isolates of the trichostrongyle mouse nematode Heligmosomoides polygyrus

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-12 DOI:10.1017/s0022149x00700903

M.A. Abu-Madi, S.N. Mohd-Zain, J.W. Lewis, A.P. Reid

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Abstract

PCR-RFLP techniques have been used to characterize wild and laboratory isolates of the trichostrongyle nematode Heligmosomoides polygyrus from the wood mouse Apodemus sylvaticus and the laboratory mouse Mus musculus respectively. Both isolates can be distinguished by eight endonuclease digestions of the ITS region of the rDNA repeat namely, Alu I, Dde I, Hpa II, Hae III, Hinf I, Hha I, Pvu II and Sal I. In two of the digests, Hinf I and Rsa I, a minor polymorphism was observed in the wild isolate of H. polygyrus which has been cultured in laboratory-bred A. sylvaticus for several generations when compared with H. p. polygyrus from wild A. sylvaticus. A minor polymorphism was also identified in further wild isolates of H. polygyrus collected from A. sylvaticus in a field site in Egham, Surrey. However no evidence of polymorphism was observed in the laboratory isolate of H. polygyrus from the CD1 strain of M. musculus and the laboratory-bred A. sylvaticus. Reasons for this are discussed and further studies on the population genetics of H. polygyrus are suggested.

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三疣小鼠线虫（Heligmosomoides polygyrus）实验室和野生分离株的基因组变异性

利用PCR-RFLP技术，对分别从木鼠（Apodemus sylvaticus）和实验鼠（Mus musculus）体内分离出的野生和实验室三刺绒毛线虫（Heligmosomoides polygyrus）进行了鉴定。这两种分离物可以通过对 rDNA 重复的 ITS 区域进行八种内切酶消化来区分，即 Alu I、Dde I、Hpa II、Hae III、Hinf I、Hha I、Pvu II 和 Sal I。在 Hinf I 和 Rsa I 这两个消化物中，与来自野生 A. sylvaticus 的 H. p. polygyrus 相比，在实验室培育的 A. sylvaticus 中培养了数代的 H. polygyrus 野生分离物中观察到了轻微的多态性。在萨里郡埃格姆的一个野外地点从A. sylvaticus中采集的H. polygyrus野生分离物中也发现了一个小的多态性。然而，在从麝鼠 CD1 株系和实验室培育的 A. sylvaticus 中分离出的 H. polygyrus 实验室分离物中，没有观察到多态性的证据。本文讨论了出现这种情况的原因，并建议进一步研究多角猴的种群遗传学。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.