Improved methods for recovering eggs of Toxocara canis from soil

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-12 DOI:10.1017/s0022149x00701131

M.R. Ruiz de Ybáñez, M. Garijo, M. Goyena, F.D. Alonso

引用次数: 0

Abstract

The ingestion of soil in parks and public places containing eggs of Toxocara may constitute a significant health risk, particularly to children. To determine the most efficient method for extracting eggs from experimentally contaminated soil, two consecutive studies were undertaken. Four techniques, including washing, sieving, vacuum, and the one recommended by the World Health Organization, were evaluated. Recovery rates of over 85% were recorded with both washing and sieving methods. Using the washing technique, all combinations of the four pre-treatment solutions, distilled water, acetoacetic solution pH 5, 0.1 N sodium hydroxide and 1% Tween 20, and seven flotation fluids with different specific gravities (S.G.) ranging from 1.20 to 1.35 were assayed. The association of distilled water and saccharose solution with an S.G. of 1.27 showed the best results, with a recovery rate of 99.91%.

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改进从土壤中回收犬弓形虫卵的方法

在公园和公共场所摄入含有弓形虫卵的土壤可能对健康构成重大威胁，尤其是对儿童。为了确定从受实验污染的土壤中提取虫卵的最有效方法，我们连续进行了两项研究。对四种技术进行了评估，包括洗涤、筛分、真空和世界卫生组织推荐的技术。洗涤法和筛分法的回收率都超过了 85%。利用洗涤技术，对四种预处理溶液（蒸馏水、pH 值为 5 的乙酰乙酸溶液、0.1 N 氢氧化钠和 1%吐温 20）和七种比重（S.G.）从 1.20 到 1.35 不等的浮选液的所有组合进行了检测。S.G. 为 1.27 的蒸馏水和糖溶液的结合效果最好，回收率达 99.91%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.