cAMP-Mediated CREM-MITF-TYR Axis Regulates Melanin Synthesis in Pacific Oysters

IF 2.6 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Marine Biotechnology Pub Date : 2024-04-13 DOI:10.1007/s10126-024-10309-9

Kunyin Jiang, Hong Yu, Lingfeng Kong, Shikai Liu, Qi Li

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Abstract

Colorful shells in bivalves are mostly caused by the presence of biological pigments, among which melanin is a key component in the formation of shell colours. Cyclic adenosine monophosphate (cAMP) is an important messenger in the regulation of pigmentation in some species. However, the role of cAMP in bivalve melanogenesis has not yet been reported. In this study, we performed in vitro and in vivo experiments to determine the role of cAMP in regulating melanogenesis in Pacific oysters. Besides, the function of cAMP-responsive element modulator (CREM) and the interactions between CREM and melanogenic genes were investigated. Our results showed that a high level of cAMP promotes the expression of melanogenic genes in Pacific oysters. CREM controls the expression of the MITF gene under cAMP regulation. In addition, CREM can regulate melanogenic gene expression, tyrosine metabolism, and melanin synthesis. These results indicate that cAMP plays an important role in the regulation of melanogenesis in Pacific oysters. CREM is a key transcription factor in the oyster melanin synthesis pathway, which plays a crucial role in oyster melanin synthesis through a cAMP-mediated CREM-MITF-TYR axis.

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cAMP 介导的 CREM-MITF-TYR 轴调节太平洋牡蛎的黑色素合成

双壳类动物的彩色贝壳主要是由生物色素造成的，其中黑色素是形成贝壳颜色的关键成分。环磷酸腺苷（cAMP）是某些物种调节色素沉积的重要信使。然而，cAMP 在双壳类动物黑色素生成过程中的作用尚未见报道。在本研究中，我们通过体外和体内实验确定了cAMP在调控太平洋牡蛎黑色素生成中的作用。此外，我们还研究了cAMP反应元件调节器（CREM）的功能以及CREM与黑色素生成基因之间的相互作用。结果表明，高水平的cAMP能促进太平洋牡蛎黑色素生成基因的表达。在cAMP的调控下，CREM控制着MITF基因的表达。此外，CREM还能调控黑色素生成基因的表达、酪氨酸代谢和黑色素合成。这些结果表明，cAMP 在调控太平洋牡蛎的黑色素生成过程中发挥着重要作用。CREM是牡蛎黑色素合成途径中的一个关键转录因子，它通过cAMP介导的CREM-MITF-TYR轴在牡蛎黑色素合成过程中发挥关键作用。

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来源期刊

Marine Biotechnology 工程技术-海洋与淡水生物学

CiteScore

4.80

自引率

3.30%

发文量

审稿时长

2 months

期刊介绍： Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.