Circulating Immune Landscape Profiling in Psoriasis Vulgaris and Psoriatic Arthritis by Mass Cytometry

IF 3.5 3区 医学 Q2 IMMUNOLOGY
Xudong Sang, Tian Gan, Gai Ge, Dan Li, Youming Mei, Chun Pan, Siyu Long, Bibo Xie, Xiaobing Yu, Zhiming Chen, Hongsheng Wang
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Abstract

Background. Psoriasis, a systemic disorder mediated by the immune system, can appear on the skin, joints, or both. Individuals with cutaneous psoriasis (PsC) have an elevated risk of developing psoriatic arthritis (PsA) during their lifetime. Despite this known association, the cellular and molecular mechanisms underlying this progression remain unclear. Methods. We performed high-dimensional, in-depth immunophenotyping of peripheral blood mononuclear cells (PBMCs) in patients with PsA and psoriasis vulgaris (PsV) by mass cytometry. Blood samples were collected before and after therapy for a longitudinal study. Then three sets of comparisons were made here: active PsA vs. active PsV, untreated PsV vs. treated PsV, and untreated PsA vs. treated PsA. Results. Marked differences were observed in multiple lymphocyte subsets of PsA related to PsV, with expansion of CD4+ T cells, CD16 NK cells, and B cells. Notably, two critical markers, CD28 and CD127, specifically differentiated PsA from PsV. The expression levels of CD28 and CD127 on both Naïve T cells (TN) and central memory CD4+ T cells (TCM) were considerably higher in PsA than PsV. Meanwhile, after treatment, patients with PsV had higher levels of CD28hi CD127hi CD4+ TCM cells, CD28hi CD127hi CD4+ TN cells, and CD16 NK cells. Conclusion. In the circulation of PsA patients, the TN and CD4+ TCM are characterized with more abundant CD28 and CD127, which effectively distinguished PsA from PsV. This may indicate that individuals undergoing PsV could be stratified at high risk of developing PsA based on the circulating levels of CD28 and CD127 on specific cell subsets.
利用质谱仪分析银屑病和银屑病关节炎的循环免疫图谱
背景。银屑病是一种由免疫系统介导的全身性疾病,可出现在皮肤、关节或两者部位。皮肤银屑病(PsC)患者一生中患银屑病关节炎(PsA)的风险较高。尽管存在这种已知的关联,但这种进展的细胞和分子机制仍不清楚。研究方法我们通过质谱仪对 PsA 和寻常型银屑病(PsV)患者的外周血单核细胞(PBMC)进行了高维、深入的免疫分型。在治疗前后采集血液样本进行纵向研究。然后在此进行三组比较:活跃的 PsA 与活跃的 PsV、未治疗的 PsV 与已治疗的 PsV,以及未治疗的 PsA 与已治疗的 PsA。结果。在与 PsV 相关的 PsA 多个淋巴细胞亚群中观察到了明显的差异,CD4+ T 细胞、CD16- NK 细胞和 B 细胞都出现了扩增。值得注意的是,CD28 和 CD127 这两个关键标志物能将 PsA 与 PsV 区分开来。在 PsA 中,CD28 和 CD127 在新生 T 细胞(TN)和中心记忆 CD4+ T 细胞(TCM)上的表达水平都大大高于 PsV。同时,经过治疗后,PsV 患者的 CD28hi CD127hi CD4+ TCM 细胞、CD28hi CD127hi CD4+ TN 细胞和 CD16- NK 细胞水平更高。结论在 PsA 患者的血液循环中,TN 和 CD4+ TCM 的 CD28 和 CD127 含量更高,这有效区分了 PsA 和 PsV。这可能表明,根据特定细胞亚群循环中的 CD28 和 CD127 水平,可对 PsV 患者进行 PsA 高风险分层。
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来源期刊
CiteScore
6.90
自引率
2.40%
发文量
423
审稿时长
15 weeks
期刊介绍: Journal of Immunology Research is a peer-reviewed, Open Access journal that provides a platform for scientists and clinicians working in different areas of immunology and therapy. The journal publishes research articles, review articles, as well as clinical studies related to classical immunology, molecular immunology, clinical immunology, cancer immunology, transplantation immunology, immune pathology, immunodeficiency, autoimmune diseases, immune disorders, and immunotherapy.
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