Localization of a 24-kDa collagenase in the Gymnorhynchus gigas plerocercoid

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-12 DOI:10.1017/s0022149x00700885

C. Vázquez-López, C. de Armas-Serra, J. Pérez-Serrano, C. Giménez-Pardo, F. Rodríguez-Caabeiro

引用次数: 0

Abstract

A 24-kDa collagenase was localized in the Gymnorhynchus gigas plerocercoid immunohistochemically by peroxidase complex staining using polyclonal antibodies from NMRI mouse sera immunized with purified enzyme. Immunoreactivity was determined at different parts of the body (scolex, vesicle and caudal region) and mainly localized in microtriches and parenchymal tissues of the scolex and vesicle. These results, along with the absence of the enzyme in the plerocercoid excretion–secretion products, suggest that the 24-kDa collagenase is produced by parenchymal cells in the anterior region and transported to the outer regions of the worm It is possible that the enzyme plays an important role in degrading parasite tissues during the moulting process.

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巨尾鳃藻中 24 kDa 胶原酶的定位

用纯化酶免疫NMRI小鼠血清中的多克隆抗体进行过氧化物酶复合物染色，通过免疫组织化学方法确定了一种24-kDa胶原酶在千足巨鳞栉水母中的位置。免疫反应在身体的不同部位（肛门、膀胱和尾部）进行了测定，主要定位于肛门和膀胱的微管和实质组织。这些结果以及犁头虫排泄物中没有该酶的情况表明，24-kDa 胶原酶由前部的实质细胞产生，并被运输到蠕虫的外部区域。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.