A blood-based multi-omic landscape for the molecular characterization of kidney stone disease†

IF 3 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular omics Pub Date : 2024-04-16 DOI:10.1039/D3MO00261F

Weibing Pan‡, Tianwei Yun, Xin Ouyang, Zhijun Ruan, Tuanjie Zhang, Yuhao An, Rui Wang and Peng Zhu

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引用次数: 0

Abstract

Kidney stone disease (KSD, also named renal calculi, nephrolithiasis, or urolithiasis) is a common urological disease entailing the formation of minerals and salts that form inside the urinary tract, frequently caused by diabetes, high blood pressure, hypertension, and monogenetic components in most patients. 10% of adults worldwide are affected by KSD, which continues to be highly prevalent and with increasing incidence. For the identification of novel therapeutic targets in KSD, we adopted high-throughput sequencing and mass spectrometry (MS) techniques in this study and carried out an integrative analysis of exosome proteomic data and DNA methylation data from blood samples of normal and KSD individuals. Our research delineated the profiling of exosomal proteins and DNA methylation in both healthy individuals and those afflicted with KSD, finding that the overexpressed proteins and the demethylated genes in KSD samples are associated with immune responses. The consistency of the results in proteomics and epigenetics supports the feasibility of the comprehensive strategy. Our insights into the molecular landscape of KSD pave the way for a deeper understanding of its pathogenic mechanism, providing an opportunity for more precise diagnosis and targeted treatment strategies for KSD.

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用于肾结石病分子特征描述的基于血液的多组学图谱

肾结石病（KSD，又称肾结石、肾结石或泌尿系结石）是一种常见的泌尿系统疾病，是一种在尿路内形成的矿物质和盐类结石，常由糖尿病、高血压、高血脂和大多数患者的单基因遗传因素引起。全球有 10% 的成年人受到 KSD 的影响，这种疾病的发病率仍然很高，而且还在不断上升。为了确定 KSD 的新型治疗靶点，我们在这项研究中采用了高通量测序和质谱（MS）技术，并对正常人和 KSD 患者血液样本中的外泌体蛋白质组数据和 DNA 甲基化数据进行了综合分析。我们的研究描绘了健康人和 KSD 患者的外泌体蛋白质和 DNA 甲基化图谱，发现 KSD 样本中过表达的蛋白质和去甲基化的基因与免疫反应有关。蛋白质组学和表观遗传学结果的一致性证明了综合策略的可行性。我们对 KSD 分子图谱的深入研究为更深入地了解其致病机制铺平了道路，为更精确地诊断 KSD 和制定有针对性的治疗策略提供了机会。

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来源期刊

Molecular omics Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

5.40

自引率

3.40%

发文量

期刊介绍： Molecular Omics publishes high-quality research from across the -omics sciences. Topics include, but are not limited to: -omics studies to gain mechanistic insight into biological processes – for example, determining the mode of action of a drug or the basis of a particular phenotype, such as drought tolerance -omics studies for clinical applications with validation, such as finding biomarkers for diagnostics or potential new drug targets -omics studies looking at the sub-cellular make-up of cells – for example, the subcellular localisation of certain proteins or post-translational modifications or new imaging techniques -studies presenting new methods and tools to support omics studies, including new spectroscopic/chromatographic techniques, chip-based/array technologies and new classification/data analysis techniques. New methods should be proven and demonstrate an advance in the field. Molecular Omics only accepts articles of high importance and interest that provide significant new insight into important chemical or biological problems. This could be fundamental research that significantly increases understanding or research that demonstrates clear functional benefits. Papers reporting new results that could be routinely predicted, do not show a significant improvement over known research, or are of interest only to the specialist in the area are not suitable for publication in Molecular Omics.