Reduction of Typical Antibiotic Resistance Genes and Mobile Gene Elements in Sewage Sludge During Sludge Bioleaching with Acidithiobacillus ferrooxidans

IF 3 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Water, Air, & Soil Pollution Pub Date : 2024-04-15 DOI:10.1007/s11270-024-07081-w

Lixin Zhao, Xinning Gao, Xiaolong Liu, Hang Li, Yi Luo, Songyan Qin

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Abstract

Sewage sludge was considered a critical reservoir for the propagation of antibiotic resistance genes (ARGs) and its treatment and disposal were important for the environment and human health. This study investigated the efficiency of the bioleaching technology for reducing eight typical antibiotic resistance genes in municipal sewage sludge, which was an emerging environmentally friendly technology for sludge dewatering. The prevalence of forty ARGs subtypes (including two mobile gene elements, MEGs) and one 16SrRNA gene were concerned with high throughput quantitative polymerase chain reaction during sludge bioleaching and a total of sixteen ARGs subtypes (including two transposase genes) were presented in the sludge samples. These genes were significantly decreasing after bioleaching, namely, one sulfonamide resistance gene (sul2), five tetracycline resistance genes (tetA-02, tetB-01, tetG-01, tetO-01, tetX), four macrolide-lincosamide-streptogramin B (MLSB) resistance genes (ermF, mphA-01, mphA-02, lnuB-01), two β-lactam resistance genes (blaOXA1/blaOXA30, blaPSE), two aminoglycoside resistance genes [aac(6´)-Ib, strB] and two MEGs (tnpA-01, tnpA-03). This result indicated that the bioleaching technology could significantly reduce the ARGs abundance of sewage sludge and the maximum removal efficiency was sixty-eight percent (mphA-02) and other ARGs subtypes such as tetA-02, tetB-01, mphA-01 and blaOXA1/blaOXA30 were decreased over fifty percent. The characteristics of Acidithiobacillus ferrooxidans contributed to the sludge bioleaching and may influence the diversity and composition of bacterial community and consequentially result in the change of ARGs abundance.

Graphical Abstract

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使用酸性硫杆菌铁氧体进行污泥生物浸出过程中减少污水污泥中的典型抗生素耐药基因和移动基因元件

污水污泥被认为是抗生素耐药基因（ARGs）繁殖的关键库，其处理和处置对环境和人类健康非常重要。本研究调查了生物浸出技术减少城市污水污泥中八种典型抗生素耐药基因的效率，这是一种新兴的环境友好型污泥脱水技术。研究利用高通量定量聚合酶链式反应对污泥生物脱水过程中的 40 个 ARGs 亚型（包括两个移动基因元件 MEG）和一个 16SrRNA 基因进行了检测，结果显示污泥样本中总共存在 16 个 ARGs 亚型（包括两个转座酶基因）。这些基因在生物浸出后明显减少，即一个磺胺抗性基因（sul2）、五个四环素抗性基因（tetA-02、tetB-01、tetG-01、tetO-01、tetX）、4 个大环内酯-林可酰胺-链霉亲和素 B（MLSB）抗性基因（ermF、mphA-01、mphA-02、lnuB-01）、2 个 β-内酰胺抗性基因（blaOXA1/blaOXA30、blaPSE）、2 个氨基糖苷类抗性基因[aac(6´)-Ib、strB]和 2 个 MEG（tnpA-01、tnpA-03）。结果表明，生物浸出技术可显著降低污水污泥中 ARGs 的含量，最大去除率为 68%（mphA-02），其他 ARGs 亚型如 tetA-02、tetB-01、mphA-01 和 blaOXA1/blaOXA30 的去除率超过 50%。酸性硫杆菌铁氧体的特性促进了污泥的生物浸出，并可能影响细菌群落的多样性和组成，从而导致 ARGs 丰度的变化。

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来源期刊

Water, Air, & Soil Pollution 环境科学-环境科学

CiteScore

4.50

自引率

6.90%

发文量

448

审稿时长

2.6 months

期刊介绍： Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments. Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation. Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.