Investigation of variability in the matrix effect on stable isotope-labeled internal standards in liquid chromatography-tandem mass spectrometry analysis of 25 pesticides in vegetables

IF 1.5 4区农林科学 Q2 ENTOMOLOGY

Journal of Pesticide Science Pub Date : 2024-03-30 DOI:10.1584/jpestics.d23-060

Arisa Banno, Yoshinori Yabuki, Motohiro Sonoda, Shinji Tanimori

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Abstract

The matrix effects (ME) in simultaneous analysis of pesticide residue using liquid chromatography-tandem mass spectrometry (LC-MS/MS) were evaluated by comparing the slopes of matrix-matched and reagent-only calibrations of four types of vegetable samples. Both the sampling and measurement variances of the ME were also determined using one-way analysis of variance. Substantial ion suppression (ME<−20%) was observed in komatsuna, spinach, and tomato when a modified Japanese official method was implemented. The ME magnitude varied significantly due to sample variability for some pesticides, but it varied by no more than 4% as a result of analytical procedure variance. This study also showed that the addition of stable isotope-labeled internal standards at low concentrations improved the recovery of pesticides from samples at various residue levels. The findings of this study highlight the importance and practical application of internal standards and the matrix-matched calibration method in residue analysis using LC-MS/MS.

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液相色谱-串联质谱法分析蔬菜中 25 种农药时基质效应对稳定同位素标记内标物影响的变异性研究

通过比较四种蔬菜样品的基质匹配校准斜率和纯试剂校准斜率，评估了液相色谱-串联质谱法（LC-MS/MS）同步分析农药残留的基质效应（ME）。此外，还使用单因子方差分析确定了 ME 的采样和测量方差。采用修改后的日本官方方法，在小松茸、菠菜和番茄中观察到了大量的离子抑制（ME<-20%）。某些农药的 ME 值因样品差异而变化很大，但因分析程序差异而变化的 ME 值不超过 4%。这项研究还表明，添加低浓度的稳定同位素标记内标可提高不同残留水平样品中农药的回收率。本研究的结果突显了内标和基质匹配校准法在使用 LC-MS/MS 进行残留分析中的重要性和实际应用。

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来源期刊

Journal of Pesticide Science 农林科学-昆虫学

CiteScore

4.30

自引率

4.20%

发文量

审稿时长

18-36 weeks

期刊介绍： The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.