Antibacterial, biocompatible, and mineralization-inducing properties of calcium silicate-based cements

IF 2.3 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

International journal of paediatric dentistry Pub Date : 2024-04-11 DOI:10.1111/ipd.13185

Taimy Cruz Hondares, Xiaoxiao Hao, Yanfang Zhao, Yuyin Lin, Dobrawa Napierala, Janice G. Jackson, Ping Zhang

{"title":"Antibacterial, biocompatible, and mineralization-inducing properties of calcium silicate-based cements","authors":"Taimy Cruz Hondares, Xiaoxiao Hao, Yanfang Zhao, Yuyin Lin, Dobrawa Napierala, Janice G. Jackson, Ping Zhang","doi":"10.1111/ipd.13185","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Different pulp capping materials have different origins and compositions, require different preparations, and may vary in their bioactive properties.</p>\n </section>\n \n <section>\n \n <h3> Aim</h3>\n \n <p>The purpose of this study was to evaluate the antibacterial activity, biocompatibility, and mineralization-inducing potential of calcium silicate-based pulp capping materials.</p>\n </section>\n \n <section>\n \n <h3> Design</h3>\n \n <p>Six contemporary calcium silicate-based cements, ProRoot MTA, MTA Angelus, Biodentine, EndoSequence, NeoMTA 2, and NeoPutty, were evaluated. The antibacterial effects of these materials against <i>Streptococcus mutans</i> UA159 and <i>Enterococcus faecalis</i> ATCC 29212 were determined by the agar diffusion assay and the direct culture test. The biocompatibility and mineralization-inducing potential of these materials in preodontoblastic 17IIA11 cells were evaluated by the MTT assay and by Alizarin Red S staining, respectively.</p>\n </section>\n \n <section>\n \n <h3> Results and Conclusion</h3>\n \n <p>In agar diffusion test, only Biodentine showed distinct antibacterial effects against <i>S. mutans</i>. All the tested materials, however, showed antibacterial effects against <i>S. mutans</i> and <i>E. faecalis</i> in the direct culture test, with Biodentine showing the strongest growth inhibition against both <i>S. mutans</i> and <i>E. faecalis</i>. All the tested materials showed acceptable biocompatibility and mineralization-supporting potential in our experimental conditions. In summary, ProRoot MTA, MTA Angelus, Biodentine, EndoSequence, NeoMTA 2, and NeoPutty demonstrated acceptable in vitro antimicrobial, biocompatible, and mineralization-supporting properties.</p>\n </section>\n </div>","PeriodicalId":14268,"journal":{"name":"International journal of paediatric dentistry","volume":"34 6","pages":"843-852"},"PeriodicalIF":2.3000,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ipd.13185","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of paediatric dentistry","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/ipd.13185","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Different pulp capping materials have different origins and compositions, require different preparations, and may vary in their bioactive properties.

Aim

The purpose of this study was to evaluate the antibacterial activity, biocompatibility, and mineralization-inducing potential of calcium silicate-based pulp capping materials.

Design

Six contemporary calcium silicate-based cements, ProRoot MTA, MTA Angelus, Biodentine, EndoSequence, NeoMTA 2, and NeoPutty, were evaluated. The antibacterial effects of these materials against Streptococcus mutans UA159 and Enterococcus faecalis ATCC 29212 were determined by the agar diffusion assay and the direct culture test. The biocompatibility and mineralization-inducing potential of these materials in preodontoblastic 17IIA11 cells were evaluated by the MTT assay and by Alizarin Red S staining, respectively.

Results and Conclusion

In agar diffusion test, only Biodentine showed distinct antibacterial effects against S. mutans. All the tested materials, however, showed antibacterial effects against S. mutans and E. faecalis in the direct culture test, with Biodentine showing the strongest growth inhibition against both S. mutans and E. faecalis. All the tested materials showed acceptable biocompatibility and mineralization-supporting potential in our experimental conditions. In summary, ProRoot MTA, MTA Angelus, Biodentine, EndoSequence, NeoMTA 2, and NeoPutty demonstrated acceptable in vitro antimicrobial, biocompatible, and mineralization-supporting properties.

Abstract Image

查看原文本刊更多论文

硅酸钙基水泥的抗菌、生物相容性和矿化诱导特性

背景不同的牙髓覆盖材料有不同的来源和成分，需要不同的制备方法，其生物活性也可能不同。目的本研究的目的是评估硅酸钙基牙髓覆盖材料的抗菌活性、生物相容性和矿化诱导潜力。通过琼脂扩散试验和直接培养试验确定了这些材料对变异链球菌 UA159 和粪肠球菌 ATCC 29212 的抗菌效果。在琼脂扩散试验中，只有 Biodentine 对变异链球菌有明显的抗菌效果。然而，在直接培养试验中，所有受试材料都显示出对变异杆菌和粪大肠杆菌的抗菌效果，其中生物丹汀对变异杆菌和粪大肠杆菌的生长抑制作用最强。在我们的实验条件下，所有测试材料都表现出了可接受的生物相容性和矿化支持潜力。总之，ProRoot MTA、MTA Angelus、Biodentine、EndoSequence、NeoMTA 2 和 NeoPutty 都表现出了可接受的体外抗菌性、生物相容性和矿化支持特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International journal of paediatric dentistry 医学-小儿科

CiteScore

5.50

自引率

2.60%

发文量

审稿时长

6-12 weeks

期刊介绍： The International Journal of Paediatric Dentistry was formed in 1991 by the merger of the Journals of the International Association of Paediatric Dentistry and the British Society of Paediatric Dentistry and is published bi-monthly. It has true international scope and aims to promote the highest standard of education, practice and research in paediatric dentistry world-wide. International Journal of Paediatric Dentistry publishes papers on all aspects of paediatric dentistry including: growth and development, behaviour management, diagnosis, prevention, restorative treatment and issue relating to medically compromised children or those with disabilities. This peer-reviewed journal features scientific articles, reviews, case reports, clinical techniques, short communications and abstracts of current paediatric dental research. Analytical studies with a scientific novelty value are preferred to descriptive studies. Case reports illustrating unusual conditions and clinically relevant observations are acceptable but must be of sufficiently high quality to be considered for publication; particularly the illustrative material must be of the highest quality.