Exploring the Therapeutic Potential of Scorpion-Derived Css54 Peptide Against Candida albicans

IF 3.3 4区生物学 Q2 MICROBIOLOGY

Journal of Microbiology Pub Date : 2024-04-08 DOI:10.1007/s12275-024-00113-4

Jonggwan Park, Hyeongsun Kim, Da Dam Kang, Yoonkyung Park

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Abstract

Candida albicans (C. albicans) is one of the most common opportunistic fungi worldwide, which is associated with a high mortality rate. Despite treatment, C. albicans remains the leading cause of life-threatening invasive infections. Consequently, antimicrobial peptides (AMPs) are potential alternatives as antifungal agents with excellent antifungal activity. We previously reported that Css54, found in the venom of Centrurodies suffusus suffusus (C. s. suffusus) showed antibacterial activity against zoonotic bacteria. However, the antifungal activity of Css54 has not yet been elucidated. The objective of this study was to identify the antifungal activity of Css54 against C. albicans and analyze its mechanism. Css54 showed high antifungal activity against C. albicans. Css54 also inhibited biofilm formation in fluconazole-resistant fungi. The antifungal mechanism of action of Css54 was investigated using membrane-related assays, including the membrane depolarization assay and analysis of the membrane integrity of C. albicans after treatment with Css54. Css54 induced reactive oxygen species (ROS) production in C. albicans, which affected its antifungal activity. Our results indicate that Css54 causes membrane damage in C. albicans, highlighting its value as a potential therapeutic agent against C. albicans infection.

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探索蝎子提取的 Css54 肽对白色念珠菌的治疗潜力

白色念珠菌（C. albicans）是全球最常见的机会性真菌之一，死亡率很高。尽管进行了治疗，但白念珠菌仍是危及生命的侵入性感染的主要原因。因此，抗菌肽（AMPs）是抗真菌剂的潜在替代品，具有卓越的抗真菌活性。我们以前曾报道过，在 Centrurodies suffusus suffusus（C. s. suffusus）毒液中发现的 Css54 对人畜共通感染的细菌具有抗菌活性。然而，Css54 的抗真菌活性尚未得到阐明。本研究的目的是确定 Css54 对白僵菌的抗真菌活性并分析其机制。Css54 对白僵菌具有很高的抗真菌活性。Css54 还能抑制氟康唑耐药真菌的生物膜形成。Css54 的抗真菌作用机制是通过与膜相关的实验进行研究的，包括膜去极化实验和白僵菌经 Css54 处理后的膜完整性分析。Css54 会诱导白僵菌产生活性氧（ROS），从而影响其抗真菌活性。我们的研究结果表明，Css54 会导致白僵菌的膜损伤，这凸显了它作为一种潜在的治疗白僵菌感染的药物的价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Microbiology 生物-微生物学

CiteScore

5.70

自引率

3.30%

发文量

审稿时长

3 months

期刊介绍： Publishes papers that deal with research on microorganisms, including archaea, bacteria, yeasts, fungi, microalgae, protozoa, and simple eukaryotic microorganisms. Topics considered for publication include Microbial Systematics, Evolutionary Microbiology, Microbial Ecology, Environmental Microbiology, Microbial Genetics, Genomics, Molecular Biology, Microbial Physiology, Biochemistry, Microbial Pathogenesis, Host-Microbe Interaction, Systems Microbiology, Synthetic Microbiology, Bioinformatics and Virology. Manuscripts dealing with simple identification of microorganism(s), cloning of a known gene and its expression in a microbial host, and clinical statistics will not be considered for publication by JM.