Facilitatory effect of low-pulse repetition frequency ultrasound on release of extracellular vesicles from cultured myotubes

IF 1.9 4区 医学 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Xiaoqi Ma, Atomu Yamaguchi, Noriaki Maeshige, Kento Tanida, Mikiko Uemura, Fuwen Lu, Hiroyo Kondo, Hidemi Fujino
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引用次数: 0

Abstract

Purpose

Extracellular vesicles (EVs) serve as carriers of intracellular factors with therapeutic effects, including tissue regeneration and attenuation of inflammatory responses. The majority of EVs in vivo are derived from skeletal muscle, which is reported to have anti-inflammatory effects. While high-intensity pulsed ultrasound (US) irradiation has been shown to promote EV secretion from myotubes, the impact of pulse repetition frequency, a US parameter affecting pulse length, on EV release remains unclear. This study aimed to investigate the impact of pulse repetition frequency of US on the release of EVs from myotubes.

Methods

C2C12 myoblasts were used in this study. After differentiation into C2C12 myotubes, US was performed for 5 min at an intensity of 3.0 W/cm2, duty cycle of 20%, acoustic frequency of 1 MHz, and different pulse repetition frequencies (100 Hz, 10 Hz, or 1 Hz). After 12 h, EVs and cells were collected for subsequent analyses.

Results

US did not cause a reduction in cell viability across all US groups compared to the control. The concentration of EVs was significantly higher in all US groups compared to the control group. In particular, the highest increase was observed in the 1-Hz group on EV concentration as well as intracellular Ca2+ level.

Conclusion

This study investigated the effect of three different pulse repetition frequencies of US on the release of EVs from cultured myotubes. It is concluded that a low-pulse repetition frequency of 1 Hz is the most effective for enhancing EV release from cultured myotubes with pulsed ultrasound.

Abstract Image

低脉冲重复频率超声波对培养肌管释放细胞外囊泡的促进作用
目的细胞外囊泡(EVs)是细胞内因子的载体,具有治疗作用,包括组织再生和减轻炎症反应。体内的大多数EVs来自骨骼肌,据报道骨骼肌具有抗炎作用。虽然高强度脉冲超声(US)辐照已被证明能促进肌管分泌EV,但影响脉冲长度的US参数--脉冲重复频率对EV释放的影响仍不清楚。本研究旨在探讨 US 脉冲重复频率对肌管释放 EV 的影响。在分化成 C2C12 肌细胞后,以 3.0 W/cm2 的强度、20% 的占空比、1 MHz 的声频和不同的脉冲重复频率(100 Hz、10 Hz 或 1 Hz)进行 US 5 分钟。12 小时后,收集 EVs 和细胞进行后续分析。与对照组相比,所有 US 组的 EVs 浓度都明显升高。结论 本研究调查了三种不同脉冲重复频率的 US 对培养肌管释放 EVs 的影响。结论是 1 Hz 的低脉冲重复频率对脉冲超声增强培养肌管中 EV 的释放最有效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.30
自引率
11.10%
发文量
102
审稿时长
>12 weeks
期刊介绍: The Journal of Medical Ultrasonics is the official journal of the Japan Society of Ultrasonics in Medicine. The main purpose of the journal is to provide forum for the publication of papers documenting recent advances and new developments in the entire field of ultrasound in medicine and biology, encompassing both the medical and the engineering aspects of the science.The journal welcomes original articles, review articles, images, and letters to the editor.The journal also provides state-of-the-art information such as announcements from the boards and the committees of the society.
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