Fiber-optic probes for real-time pH monitoring†

IF 3.5 Q2 CHEMISTRY, ANALYTICAL
Mohamed Elsherif, Fahad Alam, Ahmed E. Salih, Xinyu Wang, Peter R. Corridon, Khalil B. Ramadi and Haider Butt
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Abstract

While pH determination is a commonplace laboratory practice, conventional commercial pH probes exhibit drawbacks of bulkiness, slow response times, and signal drift. These become particularly limiting in specialized fields like tissue engineering and bio-industrial processing, where unique pH probe specifications surpass the capabilities of standard laboratory equipment. Here, we present the development of compact pH fiber probes by integrating silica optical fiber with a colorimetric pH indicator. Our approach involves cross-linking the pH indicator with a biocompatible synthetic hydrogel matrix, facilitating colorimetric and precise pH measurements. Two distinct designs of optical fiber sensors were devised to cater to a broad spectrum of applications. The first design involved attaching the hydrogel sensor to the fiber tip during the photopolymerization process, while the second design was crafted by folding the hydrogel sensor onto the bare terminal of the fiber using the casting process. The fiber sensor exhibited high sensitivity (17 nm pH−1) within physiological and pathophysiological pH ranges (6–8) when tested in reflection configuration. Validation of the developed fiber sensors was carried out on cancerous tissue phantoms derived from an ovine extracellular matrix. The unique specifications of these fiber sensors position them as promising candidates for applications in tissue engineering, cell growth, and continuous blood pH monitoring.

Abstract Image

Abstract Image

用于实时 pH 值监测的光纤探头
虽然 pH 值测定是一种常见的实验室操作,但传统的商用 pH 探针存在体积大、响应时间慢和信号漂移等缺点。在组织工程和生物工业加工等专业领域,这些缺点变得尤为突出,因为在这些领域,独特的 pH 探针规格超出了标准实验室设备的能力。在此,我们介绍了通过将二氧化硅光纤与比色 pH 指示器集成而开发出的紧凑型 pH 光纤探头。我们的方法是将 pH 指示剂与生物相容性合成水凝胶基质交联,从而实现比色和精确的 pH 测量。我们设计了两种不同的光纤传感器,以满足广泛的应用需求。第一种设计是在光聚合过程中将水凝胶传感器附着在光纤顶端,而第二种设计则是通过浇铸工艺将水凝胶传感器折叠到光纤的裸端。在反射配置测试中,纤维传感器在生理和病理生理 pH 值范围(6-8)内表现出较高的灵敏度(17 nm pH-1)。开发的纤维传感器在从绵羊细胞外基质中提取的癌症组织模型上进行了验证。这些纤维传感器的独特规格使其有望应用于组织工程、细胞生长和连续血液 pH 值监测。
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CiteScore
2.30
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0.00%
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