Large-scale validation of a plasmonic sensor for SARS-CoV-2 pseudo-neutralization with a cohort of food and retail workers†

IF 3.5 Q2 CHEMISTRY, ANALYTICAL
Julien Coutu, Pierre Ricard, Abdelhadi Djaïleb, Étienne Lavallée, Henintsoa Rabezanahary, Matthew Stuible, Yves Durocher, Caroline Gilbert, Nicholas Brousseau, Kim Santerre, Mathieu Thériault, Sylvie Trottier, Denis Boudreau, Marc-André Langlois, Joelle N. Pelletier, Mariana Baz and Jean-Francois Masson
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Abstract

Plasmonic sensors are candidates for numerous clinical applications, but few examples demonstrate their performance on large sample cohorts, a necessary step for clinical translation. The COVID-19 pandemic provided an unprecedented opportunity to validate a surface plasmon resonance (SPR) sensor for SARS-CoV-2 inhibition with a cohort of over 1000 clinical samples from the longitudinal study of a food and retail worker population. The SPR sensor provided an in vitro model to assess the level of neutralizing antibodies by measuring the inhibition of the SARS-CoV-2 spike protein interaction with ACE-2 following exposure of the spike protein to naive and immune sera (from vaccination and/or infection). In conjunction with population data on vaccination and infection, and epidemiological data from the local jurisdiction of the study cohort, it is shown that the SPR sensor performed well in assessing the level of “pseudo-neutralization” of participant sera and that the response of the SPR sensor correlates (r = 0.74) with a live virus microneutralization assay as well as with metadata of relevant events (vaccination, waves of infection, etc.) that occurred during the study period. Using these data, the article details the challenges and opportunities of using plasmonic sensors in clinical practice.

Abstract Image

Abstract Image

利用食品和零售业工人群体大规模验证 SARS-CoV-2 伪中性的质子传感器
等离子体传感器是众多临床应用的候选材料,但很少有实例能证明它们在大样本群中的性能,而这是临床转化的必要步骤。COVID-19 大流行提供了一个前所未有的机会,通过对食品和零售业工人群体的纵向研究,利用超过 1000 份临床样本对抑制 SARS-CoV-2 的表面等离子体共振 (SPR) 传感器进行验证。SPR 传感器提供了一个体外模型,通过测量 SARS-CoV-2 尖峰蛋白与 ACE-2 相互作用的抑制作用,评估中和抗体的水平。结合有关疫苗接种和感染的人口数据以及研究队列所在地的流行病学数据,结果表明 SPR 传感器在评估参与者血清的 "假性中和 "水平方面表现良好,而且 SPR 传感器的反应与活病毒微中和测定以及研究期间发生的相关事件(疫苗接种、感染浪潮等)的元数据相关(r = 0.74)。通过这些数据,文章详细介绍了在临床实践中使用电浆传感器所面临的挑战和机遇。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
2.30
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