A large presence/absence variation in the promotor of the ClLOG gene determines trichome elongation in watermelon

IF 4.1 1区农林科学 Q1 AGRONOMY

Theoretical and Applied Genetics Pub Date : 2024-04-09 DOI:10.1007/s00122-024-04601-4

Yuyuan Ma, Yu Wang, Zhiqin Zhou, Runqin Zhang, Yiru Xie, Yihan Zhang, Yongming Bo, Xiaolong Lyu, Jinghua Yang, Mingfang Zhang, Zhongyuan Hu

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引用次数: 0

Key message

The ClLOG gene encoding a cytokinin riboside 5ʹ-monophosphate phosphoribohydrolase determines trichome length in watermelon, which is associated with its promoter variations.

Abstract

Trichomes, which are differentiated from epidermal cells, are special accessory structures that cover the above-ground organs of plants and possibly contribute to biotic and abiotic stress resistance. Here, a bulked segregant analysis (BSA) of an F₂ population with significant variations in trichome length was undertaken. A 1.84-Mb candidate region on chromosome 10 was associated with trichome length. Resequencing and fine-mapping analyses indicated that a 12-kb structural variation in the promoter of Cla97C10G203450 (ClLOG) led to a significant expression difference in this gene in watermelon lines with different trichome lengths. In addition, a virus-induced gene silencing analysis confirmed that ClLOG positively regulated trichome elongation. These findings provide new information and identify a potential target gene for controlling multicellular trichome elongation in watermelon.

Abstract Image

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ClLOG 基因启动子中的大量存在/不存在变异决定了西瓜毛状体的伸长

ClLOG基因编码细胞分裂素核苷酸5ʹ-单磷酸磷酸核糖水解酶，该基因决定了西瓜毛状体的长度，而毛状体的长度与其启动子的变异有关。摘要毛状体是从表皮细胞分化出来的，是覆盖植物地上部分器官的特殊附属结构，可能有助于抗生物和非生物胁迫。本文对毛状体长度存在显著差异的 F2 群体进行了大量分离分析（BSA）。10 号染色体上一个 1.84-Mb 的候选区域与毛状体长度有关。重测序和精细图谱分析表明，Cla97C10G203450（ClLOG）启动子中的 12 kb 结构变异导致该基因在毛状体长度不同的西瓜品系中有显著的表达差异。此外，病毒诱导的基因沉默分析证实，ClLOG 对毛状体的伸长有正向调控作用。这些发现提供了新的信息，并确定了控制西瓜多细胞毛状体伸长的潜在靶基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Theoretical and Applied Genetics 生物-农艺学

CiteScore

9.60

自引率

7.40%

发文量

241

审稿时长

2.3 months

期刊介绍： Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.