Extensive evaluation of a new LC-MS/MS method to quantify monofluoroacetate toxin in the kidney

IF 2.3 3区医学 Q3 CHEMISTRY, ANALYTICAL

Journal of analytical toxicology Pub Date : 2024-04-06 DOI:10.1093/jat/bkae032

James Langston, Samuel Stump, Michael Filigenzi, Andriy Tkachenko, Jake Guag, Robert Poppenga, Wilson K Rumbeiha

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引用次数: 0

Abstract

Monofluoroacetate (MFA) is a highly lethal toxin which causes death by inhibiting cellular ATP production. The heart and brain are the primary target organs. Acute death is attributed to cardiac fibrillation and/or convulsions. Although it occurs naturally in some plants, a major source of animal intoxication is access to sodium monofluoroacetate (NaMFA) pesticide which continues to be a concern in the US and around the world despite restricted use in some countries including the US. There are also concerns about misuse of this pesticide for malicious poisoning. Currently, a tissue-based diagnostic method for NaMFA intoxication in animals is lacking. There is a critical need by the veterinary diagnostic community for a simple, sensitive, and reliable tissue-based diagnostic test to confirm NaMFA poisoning in animals. We have developed and extensively evaluated a sensitive novel LC-MS/MS method suitable for this purpose. The limits of detection (LOD) and limits of quantitation (LOQ) are 1.7 ng/g and 5.0 ng/g, respectively. The accuracy and precision met or exceeded expectations. The method performance was verified using incurred kidney obtained from animal diagnostic cases. This novel kidney-based method is now available for clinical use and can help with diagnostic purposes, including detecting potential issues related to animal foods.

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广泛评估用于定量检测肾脏中单氟乙酸酯毒素的新型 LC-MS/MS 方法

单氟乙酸酯（MFA）是一种致死率极高的毒素，它通过抑制细胞 ATP 的产生而导致死亡。心脏和大脑是主要靶器官。急性死亡的原因是心脏纤颤和/或抽搐。尽管它天然存在于某些植物中，但动物中毒的一个主要来源是接触单氟乙酸钠杀虫剂，尽管包括美国在内的一些国家限制使用单氟乙酸钠杀虫剂，但它在美国和世界各地仍是一个令人担忧的问题。人们还担心这种杀虫剂会被滥用于恶意中毒。目前，还缺乏一种基于组织的动物 NaMFA 中毒诊断方法。兽医诊断界急需一种简单、灵敏、可靠的组织诊断测试来确认动物的 NaMFA 中毒情况。为此，我们开发并广泛评估了一种灵敏的新型 LC-MS/MS 方法。该方法的检出限（LOD）和定量限（LOQ）分别为 1.7 纳克/克和 5.0 纳克/克。准确度和精密度达到或超过预期。使用从动物诊断病例中获得的肾脏对该方法的性能进行了验证。这种基于肾脏的新型方法现已可用于临床，有助于诊断目的，包括检测与动物食品相关的潜在问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of analytical toxicology 医学-毒理学

CiteScore

5.10

自引率

20.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Analytical Toxicology (JAT) is an international toxicology journal devoted to the timely dissemination of scientific communications concerning potentially toxic substances and drug identification, isolation, and quantitation. Since its inception in 1977, the Journal of Analytical Toxicology has striven to present state-of-the-art techniques used in toxicology labs. The peer-review process provided by the distinguished members of the Editorial Advisory Board ensures the high-quality and integrity of articles published in the Journal of Analytical Toxicology. Timely presentation of the latest toxicology developments is ensured through Technical Notes, Case Reports, and Letters to the Editor.