Lana Laene Lima Dias, Lázara Aline Simões Silva, Lorena Melo Vieira, Mariana Machado, Adriano Nunes-Nesi, Vinicius Coelho Kuster, Denis Coelho de Oliveira, Maurecilne Lemes da Silva, Wagner Campos Otoni, Diego Ismael Rocha
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引用次数: 0
Abstract
Sugars, hormones and plant cell wall components regulate many aspects of somatic embryogenesis which requires detailed investigations of the process. In the present study, we investigated the histology, chemical composition of the cell wall, sugar and nitrogen metabolism, and hormonal profile associated with the induction and formation of embryogenic calluses in Passiflora edulis Sims. Zygotic embryos were cultured in induction medium supplemented with 72.4 µM dichlorophenoxyacetic acid and 4.4 µM 6-benzyladenine for 30 days. The zygotic embryo consisted of a uniseriate protoderm and an undifferentiated mesophyll. After 20 days, globular-stage somatic embryos were observed at the periphery of embryogenic calluses. During callus formation, the cell wall of dividing cells showed high levels of methyl-esterified homogalacturonan (HGs) epitopes, recognized for JIM7 antibody. Epitopes of heteromannans (LM21) were identified only in the initial explants, whereas epitopes of xyloglucans (LM15) appeared throughout the morphogenetic process. Cells undergoing transdifferentiation in embryogenic calluses exhibited a drop in epitopes recognized for JIM7 and LM15 and absence of JIM5 labeling. Lipids and proteins were metabolized during early somatic embryogenesis. In contrast, hexose, starch, and amino acid concentrations increased. Hormonal dynamics were also altered: while indole-3-acetic acid and cytokinins decreased during the embryogenic process, jasmonic acid and ethylene showed the opposite trend, indicating a possible role of these stress hormones in the early somatic embryogenesis of passion fruit. These results shed light on the structural and physiological aspects of passion fruit somatic embryogenesis which may help optimize regeneration via somatic embryogenesis of this important commercial passion fruit species.
期刊介绍:
This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues.
The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.