Selective and Rapid Detection of Minocycline in Milk Using a Novel Fluorescence Conjugated Polymer Probe

IF 2.6 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2024-04-13 DOI:10.1007/s12161-024-02619-4

Lindan Gong, Lin Zhang, Hengyuan Wang, Yan Sun, Hongju Zhang, Lu-an Fan

引用次数: 0

Abstract

Minocycline is a broad-spectrum antibiotic widely used in clinical and veterinary medicine and its adverse effects are as prominent as its potent efficacy. To avoid its abuse and accumulation, it is very important to detect minocycline in medical science, food industry, and aquaculture industry. Here, a conjugated polymer probe for minocycline is prepared and its optical properties and fluorescence response to minocycline is studied. The quenching efficiency is linear relevant with the amount of minocycline at the concentration range of 0–12 µM in PBS and 0–28 µM in ethanol with the limit of detection of 0.0151 µM. Moreover, the standard addition recoveries of minocycline in skim milk samples are between 92.22 and 104.43%, and the relative standard deviation values are less than 3%. These results imply that the probe has a great potential for detecting minocycline in actual samples with simplify, rapidity, and sensitivity.

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利用新型荧光共轭聚合物探针选择性快速检测牛奶中的米诺环素

米诺环素是一种广谱抗生素，广泛应用于临床和兽医领域，其不良反应与强大的药效同样突出。为避免其滥用和蓄积，在医学、食品工业和水产养殖业中检测米诺环素非常重要。本文制备了一种米诺环素共轭聚合物探针，并研究了其光学性质和对米诺环素的荧光响应。在 PBS 中 0-12 µM 和乙醇中 0-28 µM 的浓度范围内，淬灭效率与米诺环素的量呈线性关系，检测限为 0.0151 µM。此外，脱脂奶样品中米诺环素的标准添加回收率在 92.22% 至 104.43% 之间，相对标准偏差值小于 3%。这些结果表明，该探针在检测实际样品中的米诺环素方面具有很大的潜力，而且简单、快速、灵敏。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.