Evaluation of the effect of molecular weight change of konjac glucomannan on antioxidant and tyrosinase activities

IF 2.8 4区工程技术 Q2 POLYMER SCIENCE

Macromolecular Research Pub Date : 2024-04-15 DOI:10.1007/s13233-024-00263-9

Gyu Hyun Kim, Yong Hyun Lee, Ah Young Yoo, Shaheen Amna, Jae Kweon Park

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Abstract

The purpose of this study was to characterize the biological activity of konjac glucomannan (KGM) according to its molecular weight change. KGM-degrading bacterial strain, N3 was isolated from soil and named Bacillus aerophilus following 16S rDNA sequencing and homology analysis. KGMase, a thermophilic konjac glucomannan-degrading enzyme, was partially purified by treating the culture supernatant of strain B. aerophilus N3 with 50% ammonium sulfate followed by rigorous heating at 75 °C for 30 min. The molecular weight (MW) of KGMase was determined to be approximately 41,000 by silver staining following sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). The biochemical properties stipulated that KGMase is optimally active at 45 °C and pH 6.5 which shows the high temperature and pH stability of KGMase. Manno-oligosaccharides (MOs) derived from the hydrolysis of KGM by KGMase showed a concentration-dependent increase in tyrosinase activity. MOs are bioactive compounds derived from the hydrolysis of KGM by the thermophilic KGMase of B. aerophilus and the outcomes of our study demonstrated that MOs will be suitable for application in the cosmetics industry.

Graphical abstract

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评估魔芋葡甘聚糖分子量变化对抗氧化剂和酪氨酸酶活性的影响

本研究的目的是根据魔芋葡甘聚糖（KGM）分子量的变化来描述其生物活性。通过 16S rDNA 测序和同源性分析，从土壤中分离出了降解 KGM 的细菌菌株 N3，并将其命名为嗜水气杆菌。嗜热魔芋葡甘聚糖降解酶 KGMase 的部分纯化方法是用 50%的硫酸铵处理嗜气芽孢杆菌 N3 菌株的培养上清，然后在 75 °C 下严格加热 30 分钟。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）后的银染色测定，KGMase 的分子量（MW）约为 41 000。生化特性表明，KGMase 在 45 °C、pH 值为 6.5 时具有最佳活性，这表明 KGMase 在温度和 pH 值方面具有高度稳定性。KGM酶水解KGM后产生的甘露寡糖（MOs）对酪氨酸酶活性的提高具有浓度依赖性。MOs是嗜热菌KGM酶水解KGM产生的生物活性化合物，我们的研究结果表明，MOs将适合应用于化妆品行业。

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来源期刊

Macromolecular Research 工程技术-高分子科学

CiteScore

4.70

自引率

8.30%

发文量

100

审稿时长

1.3 months

期刊介绍： Original research on all aspects of polymer science, engineering and technology, including nanotechnology Presents original research articles on all aspects of polymer science, engineering and technology Coverage extends to such topics as nanotechnology, biotechnology and information technology The English-language journal of the Polymer Society of Korea Macromolecular Research is a scientific journal published monthly by the Polymer Society of Korea. Macromolecular Research publishes original researches on all aspects of polymer science, engineering, and technology as well as new emerging technologies using polymeric materials including nanotechnology, biotechnology, and information technology in forms of Articles, Communications, Notes, Reviews, and Feature articles.