Genetic diversity and population genetic structure of Paeonia suffruticosa by chloroplast DNA simple sequence repeats (cpSSRs)

IF 5.7 1区农林科学 Q1 HORTICULTURE

Horticultural Plant Journal Pub Date : 2024-04-08 DOI:10.1016/j.hpj.2023.10.006

Qi Guo, Xian Xue, Duoduo Wang, Lixia Zhang, Wei Liu, Erqiang Wang, Xiaoqiang Cui, Xiaogai Hou

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引用次数: 0

Abstract

Andr. is an endemic shrub flower in China with 2n = 10. This study used 228 cultivars from four populations, i.e., Jiangnan, Japan, Northwest, and Zhongyuan, as materials to explore the genetic diversity levels among different populations of tree peony varieties. The results showed that 34 bands were amplified using five pairs of cpSSR primers, with an average of 6.8 bands per primer pair. The average number of different alleles (), effective alleles (), Shannon's information index (), diversity (), and polymorphic information content () were 3.600, 2.053, 0.708, 0.433, and 0.388, respectively. The value was between 0.250 and 0.500, indicating a moderate level of polymorphism for the five cpSSR primer pairs. The genetic diversity levels of peony cultivars varied among different populations, with the Northwest population showing relatively lower levels ( = 0.590, = 0.289, and = 0.263). A total of 52 haplotypes were identified in the four examined populations, and the number of haplotypes per population ranged from 11 to 22. Forty-four private haplotypes were detected across populations, and the Northwest population exhibiting the highest count of private haplotypes with 17. The mean number of effective number of haplotypes (), haplotypic richness (), and diversity () were 8.351, 6.824, and 0.893, respectively. Analysis of molecular variance indicated that genetic variation within tree peony germplasm was greater than that between germplasm resources, and the main variation was found within individuals of peony germplasm. Cluster analysis, principal coordinate analysis, and genetic structure analysis classified tree peonies from different origins into two groups, indicating a certain degree of genetic differentiation among these four tree peony cultivation groups. This study provides a theoretical basis for the exploration, utilization, and conservation of peony germplasm resources, as well as for research on the breeding of excellent varieties.

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通过叶绿体 DNA 简单序列重复序列（cpSSRs）分析芍药的遗传多样性和种群遗传结构

牡丹是中国特有的灌木花卉，2n = 10。本研究以江南、日本、西北和中原 4 个居群的 228 个栽培品种为材料，探讨不同居群牡丹品种间的遗传多样性水平。结果表明，使用 5 对 cpSSR 引物共扩增出 34 条带，平均每对引物扩增出 6.8 条带。平均等位基因数（）、有效等位基因数（）、香农信息指数（）、多样性（）和多态信息含量（）分别为 3.600、2.053、0.708、0.433 和 0.388。该值介于 0.250 和 0.500 之间，表明 5 对 cpSSR 引物的多态性处于中等水平。牡丹栽培品种的遗传多样性水平在不同种群之间存在差异，西北种群的水平相对较低（=0.590、=0.289 和=0.263）。在所考察的四个种群中，共发现了 52 个单倍型，每个种群的单倍型数量从 11 个到 22 个不等。在各个种群中发现了 44 个私有单倍型，其中西北种群的私有单倍型数量最多，为 17 个。有效单倍型平均数（）、单倍型丰富度（）和多样性（）分别为 8.351、6.824 和 0.893。分子方差分析结果表明，牡丹种质资源内部的遗传变异大于种质资源之间的遗传变异，且主要变异存在于牡丹种质资源的个体内部。聚类分析、主坐标分析和遗传结构分析将不同产地的树牡丹分为两组，表明这四个树牡丹栽培组之间存在一定程度的遗传分化。该研究为牡丹种质资源的发掘、利用和保护，以及优良品种的培育研究提供了理论依据。

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来源期刊

Horticultural Plant Journal Environmental Science-Ecology

CiteScore

9.60

自引率

14.00%

发文量

293

审稿时长

33 weeks

期刊介绍： Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.