Metabolic profiling of peri-implant crevicular fluid in peri-implantitis

IF 4.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical Oral Implants Research Pub Date : 2024-04-16 DOI:10.1111/clr.14270

Lu Song, Hongye Lu, Jimin Jiang, Antian Xu, Yanli Huang, Jia-Ping Huang, Pei-Hui Ding, Fuming He

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引用次数: 0

Abstract

Objects

This study aims to explore the etiology of peri-implantitis by comparing the metabolic profiles in peri-implant crevicular fluid (PICF) from patients with healthy implants (PH) and those with peri-implantitis (PI).

Materials and Methods

Fifty-six patients were enrolled in this cross-sectional study. PICF samples were collected and analyzed using both non-targeted and targeted metabolomics approaches. The relationship between metabolites and clinical indices including probing depth (PD), bleeding on probing (BOP), and marginal bone loss (MBL) was examined. Additionally, submucosal microbiota was collected and analyzed using 16S rRNA gene sequencing to elucidate the association between the metabolites and microbial communities.

Results

Significant differences in metabolic profiles were observed between the PH and PI groups, with 179 distinct metabolites identified. In the PI group, specific amino acids and fatty acids were significantly elevated compared to the PH group. Organic acids including succinic acid, fructose-6-phosphate, and glucose-6-phosphate were markedly higher in the PI group, showing positive correlations with mean PD, BOP, and MBL. Metabolites that increased in the PI group positively correlated with the presence of Porphyromonas and Treponema and negatively with Streptococcus and Haemophilus.

Conclusions

This study establishes a clear association between metabolic compositions and peri-implant condition, highlighting enhanced metabolite activity in peri-implantitis. These findings open avenues for further research into metabolic mechanisms of peri-implantitis and their potential therapeutic implications.

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种植体周围炎中种植体周围缝隙液的代谢谱分析

目的本研究旨在通过比较健康种植体（PH）患者和种植体周围炎（PI）患者种植体周围缝隙液（PICF）中的代谢谱，探讨种植体周围炎的病因。采用非靶向和靶向代谢组学方法收集并分析了 PICF 样本。研究了代谢物与临床指标（包括探诊深度（PD）、探诊出血（BOP）和边缘骨质流失（MBL））之间的关系。此外，还收集了粘膜下微生物群，并使用 16S rRNA 基因测序法进行分析，以阐明代谢物与微生物群落之间的关系。结果PH 组和 PI 组之间的代谢谱存在显著差异，共发现 179 种不同的代谢物。与 PH 组相比，PI 组的特定氨基酸和脂肪酸明显升高。有机酸（包括琥珀酸、6-磷酸果糖和 6-磷酸葡萄糖）在 PI 组明显升高，与平均 PD、BOP 和 MBL 呈正相关。PI 组中增加的代谢物与卟啉单胞菌和特雷波纳菌的存在呈正相关，与链球菌和嗜血杆菌呈负相关。这些发现为进一步研究种植体周围炎的代谢机制及其潜在的治疗意义开辟了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Oral Implants Research 医学-工程：生物医学

CiteScore

7.70

自引率

11.60%

发文量

149

审稿时长

3 months

期刊介绍： Clinical Oral Implants Research conveys scientific progress in the field of implant dentistry and its related areas to clinicians, teachers and researchers concerned with the application of this information for the benefit of patients in need of oral implants. The journal addresses itself to clinicians, general practitioners, periodontists, oral and maxillofacial surgeons and prosthodontists, as well as to teachers, academicians and scholars involved in the education of professionals and in the scientific promotion of the field of implant dentistry.