Au nanoparticle-catalyzed electron transfer from ammonia-borane to Ru(NH3)63+ for sensitive biosensing

IF 1.7 4区化学

Bulletin of the Korean Chemical Society Pub Date : 2024-03-05 DOI:10.1002/bkcs.12831

Seonhwa Park, Aman Bhatia, Ponnusamy Nandhakumar, Jihyeon Kim, Haesik Yang

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Abstract

Metal nanoparticle (NP)-catalyzed electron transfer (ET) from a reducing agent to a metal complex is useful for signal amplification in biosensors. For efficient ET, the metal complex must undergo rapid outer-sphere reactions, be highly water-soluble, and effectively penetrate bio/organic layers on metal NPs. Our study identifies Ru(NH₃)₆³⁺ as well-suited for this purpose. Among reducing agents, ammonia-borane (AB) enables rapid metal NP-catalyzed ET, with Au, Pt, and Pd NPs displaying similar catalytic activities. The pseudo second-order rate constant for 20-nm Au NP-catalyzed ET from AB to Ru(NH₃)₆³⁺ (1.4 × 10⁸ M⁻¹ s⁻¹) approaches the diffusion-controlled rate constant. Despite immunoglobulin G and bovine serum albumin passively adsorbed on Au NPs, catalytic activity remains largely unaffected. Applying Au NP-catalyzed ET to prostate-specific antigen detection in human serum achieves a low detection limit of 10 pg/mL. These findings highlight the potential of Ru(NH₃)₆³⁺ and AB in designing biosensors based on rapid catalytic reaction.

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金纳米粒子催化电子从氨硼烷转移到 Ru(NH3)63+ 以实现灵敏的生物传感

金属纳米粒子（NP）催化的还原剂到金属复合物的电子转移（ET）可用于生物传感器中的信号放大。要实现高效的电子转移，金属复合物必须经历快速的外层反应，具有高度的水溶性，并能有效穿透金属纳米粒子上的生物/有机层。我们的研究发现，Ru(NH3)63+ 非常适合这一目的。在还原剂中，氨硼烷（AB）能快速催化金属 NP ET，金、铂和钯 NP 具有相似的催化活性。20 纳米 Au NP 催化 ET 从 AB 到 Ru(NH3)63+ 的伪二阶速率常数（1.4 × 108 M-1 s-1）接近扩散控制速率常数。尽管免疫球蛋白 G 和牛血清白蛋白被动吸附在 Au NP 上，但催化活性基本不受影响。将 Au NP 催化的 ET 应用于人血清中前列腺特异性抗原的检测，检测限低至 10 pg/mL。这些发现凸显了 Ru(NH3)63+ 和 AB 在设计基于快速催化反应的生物传感器方面的潜力。

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来源期刊

Bulletin of the Korean Chemical Society Chemistry-General Chemistry

自引率

23.50%

发文量

182

期刊介绍： The Bulletin of the Korean Chemical Society is an official research journal of the Korean Chemical Society. It was founded in 1980 and reaches out to the chemical community worldwide. It is strictly peer-reviewed and welcomes Accounts, Communications, Articles, and Notes written in English. The scope of the journal covers all major areas of chemistry: analytical chemistry, electrochemistry, industrial chemistry, inorganic chemistry, life-science chemistry, macromolecular chemistry, organic synthesis, non-synthetic organic chemistry, physical chemistry, and materials chemistry.